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Various lysates were subjected to SDS-PAGE followed by western blot with Actin mouse monoclonal antibody (60008-1-Ig, IgM). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. Note that a higher signal is obtained using RGAM001 as it recognizes multiple epitopes.
Various lysates were subjected to SDS-PAGE, followed by western blot with Beta-Tubulin mouse monoclonal antibody (66240-1-Ig, IgG2a). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgG2a.
Various lysates were subjected to SDS-PAGE followed by western blot with FAK mouse monoclonal antibody (66258-1-Ig, IgG2c). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgG2c.
Various lysates were subjected to SDS-PAGE followed by western blot with Gelsolin mouse monoclonal antibody (66280-1-Ig, IgA). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgA.
Various lysates were subjected to SDS-PAGE, followed by western blot with NRF1 mouse monoclonal antibody (66832-1-Ig, IgG2b). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgG2b.
Various lysates were subjected to SDS-PAGE followed by western blot with ZNF174 mouse monoclonal antibody (68426-1-Ig, IgG3). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgG3.
Various lysates were subjected to SDS-PAGE followed by western blot with EIF3E mouse monoclonal antibody (67095-1-Ig, IgG1). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgG1.
Direct ELISA was performed by coating mouse monoclonal antibodies with different isotypes followed by signal development using HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701). The result indicates that RGAM701 strongly binds to Mouse IgM and does not react with Mouse IgGs or mouse IgA.
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