Betacellulin

Purity of recombinant human Betacellulin was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human Betacellulin (HZ-1339) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved during treatment with increasing concentrations of recombinant human Betacellulin for 72hrs in defined medium. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 1 - 10ng/mL.

Proteintech Betacellulin (HZ-1339) demonstrates nearly 50-fold decrease in EC50 and much higher fold induction compared to leading competitor A. NIH/3T3 cells were serum starved during treatment with increasing concentrations of recombinant human Betacellulin for 72hrs in defined medium.Cell number was quantitatively assessed by PrestoBlue HS Cell Viability Reagent. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 1 - 10 ng/mL.

Proteintech Betacellulin (HZ-1339) demonstrates more than 100-fold decrease in EC50 and much higher fold induction compared to leading competitor B. NIH/3T3 cells were serum starved during treatment with increasing concentrations of recombinant human Betacellulin for 72hrs in defined medium. Cell number was quantitatively assessed by PrestoBlue HS Cell Viability Reagent. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 1 - 10 ng/mL.