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Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG2a anti-GNL3 antibody (67169-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 488 Kit (KFA541, green), mouse IgG2a anti-Tubulin antibody (66240-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 555 Kit (KFA542, red), mouse IgG2a anti-PAF49 antibody labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA543, magenta) and mouse IgG2a anti-Clec12a antibody labeled with FlexAble 2.0 CoraLite Plus 750 Kit (KFA544, cyan). Cell nuclei were stained with DAPI (blue). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG2a anti-GNL3 antibody (67169-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 488 Kit (KFA541, green), mouse IgG2a anti-Tubulin antibody (66240-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 555 Kit (KFA542, red), mouse IgG2a anti-PAF49 antibody labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA543, magenta) and mouse IgG2a anti-Clec12a antibody labeled with FlexAble 2.0 CoraLite Plus 750 Kit (KFA544, cyan). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG2a anti-Clec12a antibody labeled with FlexAble 2.0 CoraLite Plus 750 Kit (KFA544, cyan). Cell nuclei were stained with DAPI (blue). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
1X10^6 human PBMCs were surface co-stained with Anti-Human CD3 (65133-1-Ig, Clone:OKT3) labeled with FlexAble 2.0 CoraLite Plus 750 Kit (KFA544), and Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) labeled with FlexAble 2.0 CoraLite Plus 488 Kit (KFA521). Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface co-stained with Anti-Human CD45RO (65150-1-Ig, Clone:UCHL1) labeled with FlexAble 2.0 CoraLite Plus 750 Kit (KFA544), and Anti-Human CD45RA (65226-1-Ig, Clone:F8-11-13) labeled with FlexAble 2.0 CoraLite Plus 488 Kit (KFA521). Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface co-stained with CoraLite Plus 405 Anti-Human CD3 (CL405-65151, Clone: UCHT1), Anti-Human CD8 (65135-1-Ig, Clone:OKT8) labeled with FlexAble 2.0 CoraLite Plus 750 Kit (KFA544), and Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) labeled with FlexAble 2.0 CoraLite Plus 488 Kit (KFA521). Cells were not fixed. CD3+ lymphocytes were gated.
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Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG2a anti-GNL3 antibody (67169-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 488 Kit (KFA541, green), mouse IgG2a anti-Tubulin antibody (66240-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 555 Kit (KFA542, red), mouse IgG2a anti-PAF49 antibody labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA543, magenta) and mouse IgG2a anti-Clec12a antibody labeled with FlexAble 2.0 CoraLite Plus 750 Kit (KFA544, cyan). Cell nuclei were stained with DAPI (blue). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
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| 別包装 |
あり
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| 種由来 |
Mouse
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| 標識物 |
CoraLite(R) 750
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| 励起波長 |
755
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| 蛍光波長 |
780
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| 適用 |
Western Blot
Immuno Fluorescence
Flow Cytometry
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| 交差種 |
Mouse
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| 形状 |
液状
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| メーカー |
品番 |
包装 |
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PGI
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KFA544
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10 RXN
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