FlexAble 2.0 CoraLite(R) Plus 647 Antibody Labeling Kit for Mouse IgG2a, Trial Size

Immunofluorescence of Rat brain: FFPE Rat brain sections were stained with anti-MAP2 (17490-1-AP) labeled with FlexAble 2.0 CoraLite Plus 488 Kit (KFA501, green), anti-GFAP (60190-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 555 Kit (KFA542, orange), anti-TDP-43 (67345-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA543,magenta) and DAPI (blue).

Immunofluorescence of Rat brain: FFPE Rat brain sections were stained with anti-MAP2 (17490-1-AP) labeled with FlexAble 2.0 CoraLite Plus 488 Kit (KFA501, green), anti-GFAP (60190-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 555 Kit (KFA542, orange), anti-TDP-43 (67345-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA543,magenta) and DAPI (blue).

Immunofluorescence of Rat brain: FFPE Rat brain sections were stained with anti-MAP2 (17490-1-AP) labeled with FlexAble 2.0 CoraLite Plus 555 Kit (KFA502, orange), anti-GFAP (60190-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA543, magenta), anti-TDP-43 (67345-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 488 Kit (KFA541,green) and DAPI (blue).

Immunofluorescence of Rat brain: FFPE Rat brain sections were stained with anti-MAP2 (17490-1-AP) labeled with FlexAble 2.0 CoraLite Plus 555 Kit (KFA502, orange), anti-GFAP (60190-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA543, magenta), anti-TDP-43 (67345-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 488 Kit (KFA541,green) and DAPI (blue).

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG2a anti-GNL3 antibody (67169-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 488 Kit (KFA541, green), mouse IgG2a anti-Tubulin antibody (66240-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 555 Kit (KFA542, red), mouse IgG2a anti-PAF49 antibody labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA543, magenta) and mouse IgG2a anti-Clec12a antibody labeled with FlexAble 2.0 CoraLite Plus 750 Kit (KFA544, cyan). Cell nuclei were stained with DAPI (blue). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG2a anti-GNL3 antibody (67169-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 488 Kit (KFA541, green), mouse IgG2a anti-Tubulin antibody (66240-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 555 Kit (KFA542, red), mouse IgG2a anti-PAF49 antibody labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA543, magenta) and mouse IgG2a anti-Clec12a antibody labeled with FlexAble 2.0 CoraLite Plus 750 Kit (KFA544, cyan). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG2a anti-PAF49 antibody labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA543, magenta). Cell nuclei were stained with DAPI (blue). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

1X10^6 human PBMCs were surface co-stained with Anti-Human CD3 (65133-1-Ig, Clone:OKT3) labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA543), and Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) labeled with FlexAble 2.0 CoraLite Plus 488 Kit (KFA521). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with Anti-Human CD45RO (65150-1-Ig, Clone:UCHL1) labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA543), and Anti-Human CD45RA (65226-1-Ig, Clone:F8-11-13) labeled with FlexAble 2.0 CoraLite Plus 488 Kit (KFA521). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with CoraLite Plus 405 Anti-Human CD3 (CL405-65151, Clone: UCHT1), Anti-Human CD8 (65135-1-Ig, Clone:OKT8) labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA543), and Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) labeled with FlexAble 2.0 CoraLite Plus 488 Kit (KFA521). Cells were not fixed. CD3+ lymphocytes were gated.