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Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-B23 (60096-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 488 Kit (KFA521, green), mouse IgG1 anti-Lamin (66095-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 555 Kit (KFA522, yellow), mouse IgG1 anti-Gorasp2 (66627-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA523, magenta) and mouse IgG1 anti-HSP60 (66041-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 750 Kit (KFA524, cyan). Cell nuclei were stained with DAPI (blue). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-B23 (60096-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 488 Kit (KFA521, green), mouse IgG1 anti-Lamin (66095-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 555 Kit (KFA522, yellow), mouse IgG1 anti-Gorasp2 (66627-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA523, magenta) and mouse IgG1 anti-HSP60 (66041-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 750 Kit (KFA524, cyan). Cell nuclei were stained with DAPI (blue). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-HSP60 antibody directly conjugated to CoraLite Plus 488 (CL488-66041, green), mouse IgG1 anti-HDAC1 antibody directly conjugated to CoraLite Plus 555 (CL555-66085, red), mouse IgG1 anti-Gorasp2 antibody (66627-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA523, magenta) and mouse IgG1 anti-B23 antibody (60096-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 750 Kit (KFA524, cyan). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-HSP60 antibody directly conjugated to CoraLite Plus 488 (CL488-66041, green), mouse IgG1 anti-HDAC1 antibody directly conjugated to CoraLite Plus 555 (CL555-66085, red), mouse IgG1 anti-Gorasp2 antibody (66627-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA523, magenta) and mouse IgG1 anti-B23 antibody (60096-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 750 Kit (KFA524, cyan). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-HSP60 antibody (66041-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 750 Kit (KFA524, cyan). Cell nuclei were stained with DAPI (blue). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
1X10^6 human PBMCs were surface co-stained with CoraLite Plus 488 Anti-Human CD45RO (CL488-65150, Clone:UCHL1), and Anti-Human CD45RA (65226-1-Ig, Clone:F8-11-13) labeled with FlexAble 2.0 CoraLite Plus 750 Kit (KFA524). Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface co-stained with CoraLite Plus 488 Anti-Human CD3 (CL488-65151, Clone:UCHT1), and Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) labeled with FlexAble 2.0 CoraLite Plus 750 Kit (KFA524). Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface co-stained with CoraLite Plus 488 Anti-Human CD45 (CL488-65109, Clone:HI30), and Anti-Human CD3 (65151-1-Ig, Clone:UCHT1) labeled with FlexAble 2.0 CoraLite Plus 750 Kit (KFA524). Cells were not fixed. Lymphocytes were gated.
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Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-B23 (60096-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 488 Kit (KFA521, green), mouse IgG1 anti-Lamin (66095-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 555 Kit (KFA522, yellow), mouse IgG1 anti-Gorasp2 (66627-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA523, magenta) and mouse IgG1 anti-HSP60 (66041-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 750 Kit (KFA524, cyan). Cell nuclei were stained with DAPI (blue). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
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| 別包装 |
あり
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| 種由来 |
Mouse
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| 交差種 |
Mouse
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| 適用 |
Western Blot
Immuno Fluorescence
Flow Cytometry
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| 標識物 |
CoraLite(R) 750
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| 励起波長 |
755
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| 蛍光波長 |
780
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| メーカー |
品番 |
包装 |
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PGI
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KFA524
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50 RXN
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