FlexAble 2.0 CoraLite(R) Plus 647 Antibody Labeling Kit for Mouse IgG1

Immunofluorescence of Mouse colon: FFPE Mouse colon sections were stained with anti-FUS (11570-1-AP) labeled with FlexAble 2.0 CoraLite Plus 488 Kit (KFA501, green), anti-α-SMA (67735-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA523, magenta),anti-E-cadherin (60335-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 555 Kit (KFA562, orange) and DAPI (blue).

Immunofluorescence of Mouse colon: FFPE Mouse colon sections were stained with anti-FUS (11570-1-AP) labeled with FlexAble 2.0 CoraLite Plus 488 Kit (KFA501, green), anti-α-SMA (67735-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA523, magenta),anti-E-cadherin (60335-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 555 Kit (KFA562, orange) and DAPI (blue).

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-B23 (60096-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 488 Kit (KFA521, green), mouse IgG1 anti-Lamin (66095-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 555 Kit (KFA522, yellow), mouse IgG1 anti-Gorasp2 (66627-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA523, magenta) and mouse IgG1 anti-HSP60 (66041-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 750 Kit (KFA524, cyan). Cell nuclei were stained with DAPI (blue). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-B23 (60096-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 488 Kit (KFA521, green), mouse IgG1 anti-Lamin (66095-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 555 Kit (KFA522, yellow), mouse IgG1 anti-Gorasp2 (66627-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA523, magenta) and mouse IgG1 anti-HSP60 (66041-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 750 Kit (KFA524, cyan). Cell nuclei were stained with DAPI (blue). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-HSP60 antibody directly conjugated to CoraLite Plus 488 (CL488-66041, green), mouse IgG1 anti-HDAC1 antibody directly conjugated to CoraLite Plus 555 (CL555-66085, red), mouse IgG1 anti-Gorasp2 antibody (66627-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA523, magenta) and mouse IgG1 anti-B23 antibody (60096-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 750 Kit (KFA524, cyan). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-HSP60 antibody directly conjugated to CoraLite Plus 488 (CL488-66041, green), mouse IgG1 anti-HDAC1 antibody directly conjugated to CoraLite Plus 555 (CL555-66085, red), mouse IgG1 anti-Gorasp2 antibody (66627-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA523, magenta) and mouse IgG1 anti-B23 antibody (60096-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 750 Kit (KFA524, cyan). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-B23 antibody (60096-1-Ig) labeled with Multi-rAb CoraLite Plus 488 Goat anti-Mouse Recombinant Secondary (RGAM002, green), mouse IgG1 anti-Lamin (66095-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 555 Kit (KFA522, red) and mouse IgG1 anti-ATP5O (66696-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA523, magenta). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG1 anti-B23 antibody (60096-1-Ig) labeled with Multi-rAb CoraLite Plus 488 Goat anti-Mouse Recombinant Secondary (RGAM002, green), mouse IgG1 anti-Lamin antibody (66095-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 555 Kit (KFA522, red) and mouse IgG1 anti-ATP5O antibody (66696-1-Ig) labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA523, magenta). Cell nuclei were stained with DAPI (blue). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

1X10^6 human PBMCs were surface co-stained with CoraLite Plus 488 Anti-Human CD45RO (CL488-65150, Clone:UCHL1), and Anti-Human CD45RA (65226-1-Ig, Clone:F8-11-13) labeled with FlexAble 2.0

1X10^6 human PBMCs were surface co-stained with CoraLite Plus 488 Anti-Human CD3 (CL488-65151, Clone:UCHT1), and Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) labeled with FlexAble 2.0 CoraLite Plus 647 Kit (KFA523). Cells were not fixed. Lymphocytes were gated.