Protein A  
DyLight (商標名)

ZASC1 and ZBTB2 regulate HIV-1 gene expression. A sample of cells were harvested for total protein (see C) at this time. 48 hours post infection HIV-1 transcription was assayed for by measuring firefly luciferase and cell viability was monitored by the ATP assay CellTiter-glo (Promega). The data shown are the average mean values obtained in an experiment performed with quadruplicate samples and are representative of three independent experiments. (C and D) Western blots demonstrating loss of ZASC1 or ZBTB2 protein accumulation in (C) SUPT1 cells transfected with siRNAs targeting ZBTB2 or non-targeting (NT) siRNA or (D) ZASC1 and ZBTB2 knockout cells generated by CRISPR/CAS9 in SUPT1 and Jurkat T cell lines. (E) Co-immunoprecipitation assays of endogenous proteins. WT Jurkat, ZASC1 or ZBTB2 knockout cells (1X107) were lysed and incubated with ZASC1, ZBTB2 or non-specific (NS) rabbit IgG. Co-immunoprecipitating complexes were detected by western blotting. Fig 3. PMID: 33635925.