Anti IgG (H&L)   DyLight (商標名)

DyLight^TM dyes can be used for two-color western blot detection with low background and high signal. Anti-tubulin was detected using a DyLight^TM 680 conjugate. Anti-TNFa was detected using a DyLight^TM 800 conjugate. The image was captured using the Odyssey^(R) Infrared Imaging System developed by LI-COR.

Western Blot showing detection of Golden Syrian Hamster IgG (Lane 2) and Armenian Hamster IgG (Lane 3) was run on a 4-20% gel and transferred to 0.45 μm nitrocellulose. After blocking with 5% Blotto (p/n B501-0500) 30 min at 20C, Anti-Golden Syrian & Armenian Hamster IgG (H&L) (GOAT) Antibody DyLight^TM 800 Conjugated (p/n 620-145-440) secondary antibody was used at 1:5000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged on the LiCor Odyssey imaging system. Molecular weight markers are in lane 1.

Properties of DyLight^TM Conjugates.