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Properties of DyLightTM Conjugates.
DyLightTM dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLightTM 680 conjugate. Anti-TNFa was detected using a DyLightTM 800 conjugate. The image was captured using the OdysseyR Infrared Imaging System developed by LI-COR.
Western blot of DyLightTM 680 conjugated Donkey Anti-Sheep IgG Pre-Adsorbed secondary antibody. Lane 1: Sheep IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: DyLightTM 680 donkey secondary antibody at 1:5,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Sheep IgG. Other band(s): None.
Properties of DyLight? Conjugates.
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