Anti IgG (H&L)  
DyLight (商標名)

Western Blot using Sheep Anti-Rabbit IgG DyLight？800.TGF‐β‐mediated crosstalk between pericytes and CRC cells modulates pericyte secretome. (A) Incubation in HCT116 CM for 1？h induces SMAD3 phosphorylation in PC, as assessed by western blot. Exogenous recombinant TGF‐β (10？ng・mL−1) was used as a positive control, and β‐actin was used as loading control (n？=？3). (C) Western blot showing increased expression of αSMA in PC cocultured with HCT116 cells or stimulated with 10？ng・mL−1？TGF‐β1 for 48？h (n？=？3). α‐tubulin was used as loading control. Numbers indicate the expression fold change relative to the loading control. Fig. 5. PMID: 32767843.

Western Blot results using Sheep Anti-Rabbit IgG DyLight？800.Insulin‐like growth factor‐binding protein 3 increases CRC cell migration and invasion through Akt activation. (D) Treatment with 50？ng・mL−1？IGFBP‐3 for 72？h promotes the expression of N‐cadherin in HCT116 cells as assessed by western blot (top panel). Phosphorylation status of Akt (middle panel) and MAPK (bottom panel) in HCT116 cells treated with 50？ng・mL−1？IGFBP‐3 for 15？min. Representative images of three independent experiments (n？=？3). Numbers indicate the expression fold change relative to the loading control. Fig. 7. PMID: 32767843.

Western Blot results using Sheep Anti-Rabbit IgG DyLight？800.Effect of SFN on p73 expression and caspase enzyme activities. SW480 cells were treated with 0, 1, 5, 10, 15 and 20 μM SFN for 24 h. (A) The protein expression levels of p73, PUMA and NOXA were examined by western blotting. Quantified band densities are summarized below the images of the bands, and target protein expression levels were normalized to that of b-actin. (B) The activity of caspase-3, −7, −8 and −9 was determined using specific ELISA kits. The results of three independent experiments are shown. **P<0.01 vs. 0 μM SFN. SFN, sulforaphane; PUMA, p53 upregulated modulator of apoptosis; NOXA, phorbol-12-myristate-13-acetate-induced protein 1; ELISA, enzyme-linked immunosorbent assay. Figure？2. PMID: 28944886.

Western Blot results using Sheep Anti-Rabbit IgG DyLight？800.Effect of SFN on MAPKs and intrinsic apoptotic signaling pathways. SW480 cells were treated with 0, 1, 5, 10, 15 and 20 μM SFN for 24 h. (A) The protein expression levels of (A) p-p38, p-Erk and p-JNK and (B) Bcl-2 and Bax were examined by western blotting, and the quantified results are summarized below the images of the protein bands. Target protein expression levels were normalized to that of b-actin. (C) The Bax/Bcl-2 ratio. (D) The MMP was examined using a MMP assay kit with a JC-1 probe. The results of three independent experiments are shown. *P<0.05 and **P<0.01 vs. 0 μM SFN. SFN, sulforaphane; MAPK, mitogen-activated protein kinases; p-, phosphorylated; Erk, extracellular signal-regulated kinases; JNK, c-Jun N-terminal kinases; Bcl-2, B-cell lymphoma-2; Bax, Bcl-2-associated protein X; MMP, mitochondrial membrane potential. Figure？3. PMID: 28944886.

Western Blot Results using Sheep Anti-Rabbit IgG DyLight？800.LPL stabilizes Pyk2-ASC interaction by regulating Pyk2 localization.(C) WT BMDMs were primed with LPS and stimulated for NLRP3 activation by ATP (30 min) in the absence and presence of z-VAD-fmk (50 μM, 1 h), MCC-950 (50 μM, 1 h), and PF-431396 (25 μM) for 1 h or 4.5 h. Cell lysates were probed for Pyk2 phosphorylation and IL-1β products by immunoblotting. The density of each band is shown below. Here, β-actin is the total cell control. Figure 4. PMID: 35294888.

DyLight？ dyes can be used for two-color western blot detection with low background and high signal.？ Anti-tubulin was detected using a DyLight？ 680 conjugate.？ Anti-TNFa was detected using a DyLight？ 800 conjugate. The image was captured using the OdysseyR Infrared Imaging System developed by LI-COR.

Properties of DyLight？ Conjugates.