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(A) Bronchoalveolar lavage concentration optimization. Average BALF IgE fluorescence results for each protein group demonstrating concentration efficacy for unconcentrated, and concentrated (10‐fold) using either Amicon filter or PD10 columns/lyophilizing, A 1‐way ANOVA for each protein group using a BALF pool from 6 horses (n = 3 SEA; n = 3 control) demonstrated there was no significant difference (p > .05). Means that have no superscript in common are significantly different from each other. (B) Average BALF pool horses (n = 3 SEA; n = 3 control) IgE fluorescence results for the main protein groups at various concentrations using Amicon filtration. Groupings included arthropod, fungi, pollen and other; which largely consisted of food and environmental proteins. Significant differences of each protein group were calculated individually by 1‐way ANOVA with Tukey's HSD (* = p < .05). Means that have no superscript in common are significantly different from each other. Figure 1. PMID: 31429513.
Bland Altman plots to compare ELISA and microarray IgE with specific proteins. The acceptable level of bias is dictated by the solid line. The dashed lines represent the 95% limits of agreement as the mean difference (2 SD). Variable influences on the projection scores significant for class prediction from the environmentally mixed group of horses (n=138) after VIP selection. Sup Fig 1. PMID: 31645629.
DyLight？ dyes can be used for two-color Western Blot detection with low background and high signal.？ Anti-tubulin was detected using a DyLight？ 549 conjugate.？ Anti-TNFa was detected using a DyLight？ 649 conjugate. The image was captured using the Typhoon？ 9410 Imaging System.
Properties of DyLight？ Conjugates.
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(A) Bronchoalveolar lavage concentration optimization. Average BALF IgE fluorescence results for each protein group demonstrating concentration efficacy for unconcentrated, and concentrated (10‐fold) using either Amicon filter or PD10 columns/lyophilizing, A 1‐way ANOVA for each protein group using a BALF pool from 6 horses (n = 3 SEA; n = 3 control) demonstrated there was no significant difference (p > .05). Means that have no superscript in common are significantly different from each other. (B) Average BALF pool horses (n = 3 SEA; n = 3 control) IgE fluorescence results for the main protein groups at various concentrations using Amicon filtration. Groupings included arthropod, fungi, pollen and other; which largely consisted of food and environmental proteins. Significant differences of each protein group were calculated individually by 1‐way ANOVA with Tukey's HSD (* = p < .05). Means that have no superscript in common are significantly different from each other. Figure 1. PMID: 31429513.
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| 別品名 |
Rabbit Anti Mouse IgG1 (Gamma 1 chain) Antibody DyLight 649TM Conjugated, Rabbit Anti-Mouse IgG1 Antibody DyLight 649TM Conjugated
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| 交差種 |
Mouse
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| 免疫動物 |
Rabbit
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| 標識物 |
DyLightTM 649
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| 精製度 |
Affinity Purified
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| 参考文献 |
[Pub Med ID]31429513
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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610-443-040
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 法規制 |
毒
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| 保存温度 |
４℃
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