Anti β TrCP2  

Western blot using Rockland's affinity purified anti-bTrCP2 antibody shows detection of mouse and human bTrCP2 (arrowhead) in NIH3T3 (p/n W10-000-358) [lane 1] and 293 (p/n W09-000-365) [lane 2] whole cell lysates, respectively.？ The band appears as a 58 kDa protein, although a 62.1 kDa band is predicted.？ The identity of faint higher molecular weight bands is not known.？ The primary antibody was used at a 1:200 dilution incubated in 5% BLOTTO overnight at 4°C.？ Detection occurred using HRP conjugated Goat-anti-Rabbit IgG (p/n 611-103-122) diluted 1:20,000 in blocking buffer (p/n MB-070) for 1 h at 4 °C.