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Figure 1. Western blot analysis of TRAF2 using anti-TRAF2 antibody (PB9516).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Brain Tissue Lysate at 50ug,
Lane 2: Rat Testis Tissue Lysate at 50ug,
Lane 3: Mouse Brain Tissue Lysate at 50ug,
Lane 4: Human Placenta Tissue Lysate at 50ug,
Lane 5: 22RV1 Whole Cell Lysate at 40ug,
Lane 6: SMMC Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF2 antigen affinity purified polyclonal antibody (Catalog # PB9516) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002)？with Tanon 5200 system. A specific band was detected for TRAF2 at approximately 53 kDa. The expected band size for TRAF2 is at 53 kDa.
Figure 2. IHC analysis of TRAF2 using anti-TRAF2 antibody (PB9516).
TRAF2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TRAF2 Antibody (PB9516) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 1. Western blot analysis of TRAF2 using anti-TRAF2 antibody (PB9516).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Brain Tissue Lysate at 50ug,
Lane 2: Rat Testis Tissue Lysate at 50ug,
Lane 3: Mouse Brain Tissue Lysate at 50ug,
Lane 4: Human Placenta Tissue Lysate at 50ug,
Lane 5: 22RV1 Whole Cell Lysate at 40ug,
Lane 6: SMMC Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF2 antigen affinity purified polyclonal antibody (Catalog # PB9516) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002)？with Tanon 5200 system. A specific band was detected for TRAF2 at approximately 53 kDa. The expected band size for TRAF2 is at 53 kDa.
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| 別品名 |
TNF receptor-associated factor 2;6.3.2.-;E3 ubiquitin-protein ligase TRAF2;Tumor necrosis factor type 2 receptor-associated protein 3;TRAF2;TRAP3;
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| 種由来 |
Human
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| 交差種 |
Human
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| 適用 |
Western Blot
Immunohistochemistry
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 標識物 |
Biotin
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| 精製度 |
Affinity Purified
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| GENE ID |
7186
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| Accession No.(Gene/Protein) |
Q12933
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| Gene Symbol |
TRAF2
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| 分子量 |
55859 MW
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| 概要 |
Boster Bio Anti-TRAF2 Antibody Picoband® catalog # PB9516. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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| 参考文献 |
1. Aizawa, S., Nakano, H., Ishida, T., Horie, R., Nagai, M., Ito, K., Yagita, H., Okumura, K., Inoue, J., Watanabe, T. Tumor necrosis factor receptor-associated factor (TRAF) 5 and TRAF2 are involved in CD30-mediated NF-kappa-B activation. J. Biol. Chem. 272: 2042-2045, 1997.
2. Kanamori, M., Suzuki, H., Saito, R., Muramatsu, M., Hayashizaki, Y. T2BP, a novel TRAF2 binding protein, can activate NF-kappa-B and AP-1 without TNF stimulation. Biochem. Biophys. Res. Commun. 290: 1108-1113, 2002.
3. Rothe, M., Xiong, J., Shu, H.-B., Williamson, K., Goddard, A., Goeddel, D. V. I-TRAF is a novel TRAF-interacting protein that regulates TRAF-mediated signal transduction. Proc. Nat. Acad. Sci. 93: 8241-8246, 1996.
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| メーカー |
品番 |
包装 |
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BBT
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PB9516-BIOTIN
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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