| 別品名 |
Guanine nucleotide-binding protein subunit beta-2-like 1;Cell proliferation-inducing gene 21 protein;Guanine nucleotide-binding protein subunit beta-like protein 12.3;Human lung cancer oncogene 7 protein;HLC-7;Receptor for activated C kinase;Receptor of activated protein kinase C 1;RACK1;Guanine nucleotide-binding protein subunit beta-2-like 1, N-terminally processed;GNB2L1;HLC7, PIG21;
|
| 種由来 |
Human
|
| 標識物 |
Biotin
|
| 精製度 |
Affinity Purified
|
| 適用 |
Western Blot
Immuno Fluorescence
Immunocytochemistry (cell)
Flow Cytometry
|
| 免疫動物 |
Rabbit
|
| 交差種 |
Human
Mouse
Rat
|
| GENE ID |
10399
|
| Accession No.(Gene/Protein) |
P63244
|
| Gene Symbol |
GNB2L1
|
| 感度 |
<5pg/ml
|
| 分子量 |
35077 MW
|
| 形状 |
凍結乾燥品
|
| 参考文献 |
1. Ceci, M., Gaviraghi, C., Gorrini, C., Sala, L. A., Offenhauser, N., Marchisio, P. C., Biffo, S. Release of eIF6 (p27-BBP) from the 60S subunit allows 80S ribosome assembly. Nature 426: 579-584, 2003.
2. Patterson, R. L., van Rossum, D. B., Barrow, R. K., Snyder, S. H. RACK1 binds to inositol 1,4,5-triphosphate receptors and mediates Ca(2+) release. Proc. Nat. Acad. Sci. 101: 2328-2332, 2004.
3. Robles, M. S., Boyault, C., Knutti, D., Padmanabhan, K., Weitz, C. J. Identification of RACK1 and protein kinase C-alpha as integral components of the mammalian circadian clock. Science 327: 463-466, 2010.
|
|
※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of RACK1 using anti-RACK1 antibody (PB9243).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human Raji whole cell lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse RAW264.7 whole cell lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RACK1 antigen affinity purified polyclonal antibody (Catalog # PB9243) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RACK1 at approximately 36 kDa and for RACK1 mature form at approximately 32 kDa. The expected band size for RACK1 is at 35 kDa.
Figure 2. IF analysis of RACK1 using anti-RACK1 antibody (PB9243).
RACK1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RACK1 Antibody (PB9243) overnight at 4°C. DyLight?550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Figure 3. Flow Cytometry analysis of A431 cells using anti-RACK1 antibody (PB9243).
Overlay histogram showing A431 cells stained with PB9243 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RACK1 Antibody (PB9243,1μg/1x106 cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
|
|
|
|
Figure 1. Western blot analysis of RACK1 using anti-RACK1 antibody (PB9243).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human Raji whole cell lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse RAW264.7 whole cell lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RACK1 antigen affinity purified polyclonal antibody (Catalog # PB9243) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RACK1 at approximately 36 kDa and for RACK1 mature form at approximately 32 kDa. The expected band size for RACK1 is at 35 kDa.
|
|
|
| メーカー |
品番 |
包装 |
|
BBT
|
PB9243-BIOTIN
|
100 UG
|
※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
1週間程度
|
| 保存温度 |
-２０℃
|
|
