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Figure 1. Western blot analysis of CD82 using anti-CD82 antibody (PB9170).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human U-87MG whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD82 antigen affinity purified polyclonal antibody (Catalog # PB9170) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD82 at approximately 30-36 kDa. The expected band size for CD82 is at 30 kDa.
Figure 2. IHC analysis of CD82 using anti-CD82 antibody (PB9170).
CD82 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD82 Antibody (PB9170) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Figure 3. IHC analysis of CD82 using anti-CD82 antibody (PB9170).
CD82 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD82 Antibody (PB9170) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 1. Western blot analysis of CD82 using anti-CD82 antibody (PB9170).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human U-87MG whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD82 antigen affinity purified polyclonal antibody (Catalog # PB9170) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD82 at approximately 30-36 kDa. The expected band size for CD82 is at 30 kDa.
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| 別品名 |
CD82 antigen;C33 antigen;IA4;Inducible membrane protein R2;Metastasis suppressor Kangai-1;Suppressor of tumorigenicity 6 protein;Tetraspanin-27;Tspan-27;CD82;CD82;KAI1, SAR2, ST6, TSPAN27;
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| 種由来 |
Human
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| 交差種 |
Human
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| 適用 |
Western Blot
Immunohistochemistry
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 標識物 |
Horseradish Peroxidase
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| 精製度 |
Affinity Purified
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| GENE ID |
3732
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| Accession No.(Gene/Protein) |
P27701
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| Gene Symbol |
CD82
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| 分子量 |
29626 MW
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| 概要 |
Boster Bio Anti-CD82 Antibody Picoband® catalog # PB9170. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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| 参考文献 |
1. Dong, J.-T.; Lamb, P. W.; Rinker-Schaeffer, C. W.; Vukanovic, J.; Ichikawa, T.; Isaacs, J. T.; Barrett, J. C. : KAI1, a metastasis suppressor gene for prostate cancer on human chromosome 11p11.2. Science 268: 884-886, 1995. 2. Mashimo, T.; Watabe, M.; Hirota, S.; Hosobe, S.; Miura, K.; Tegtmeyer, P. J.; Rinker-Shaeffer, C. W.; Watabe, K. : The expression of the KAI1 gene, a tumor metastasis suppressor, is ly activated by p53. Proc. Nat. Acad. Sci. 95: 11307-11311, 1998.3. Miyazaki, T.; Kato, H.; Shitara, Y.; Yoshikawa, M.; Tajima, K.; Masuda, N.; Shouji, H.; Tsukada, K.; Nakajima, T.; Kuwano, H. : Mutation and expression of the metastasis suppressor gene KAI1 in esophageal squamous cell carcinoma. Cancer 89: 955-962, 2000. 4. Jee, B. K.; Park, K. M.; Surendran, S.; Lee, W. K.; Han, C. W.; Kim, Y. S.; Lim, Y. : KAI1/CD82 suppresses tumor invasion by MMP9 inactivation via TIMP1 up-regulation in the H1299 human lung carcinoma cell line. Biochem. Biophys. Res. Commun. 342: 655-661, 2006.
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| メーカー |
品番 |
包装 |
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BBT
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PB9170-HRP
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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