|
※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of PD-L1 using anti-PD-L1 antibody (PB9154).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: human K562 whole cell lysates,
Lane 6: human A549 whole cell lysates,
Lane 7: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PD-L1 antigen affinity purified polyclonal antibody (Catalog # PB9154) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002)？with Tanon 5200 system. A specific band was detected for PD-L1 at approximately 40-50 kDa. The expected band size for PD-L1 is at 33 kDa.
Figure 2. Flow Cytometry analysis of JK cells using anti-PD-L1 antibody (PB9154).
Overlay histogram showing JK cells stained with PB9154 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PD-L1 Antibody (PB9154, 1 μg/1x106 cells) for 30 min at 20°C. DyLightR488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
|
|
|
|
Figure 1. Western blot analysis of PD-L1 using anti-PD-L1 antibody (PB9154).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: human K562 whole cell lysates,
Lane 6: human A549 whole cell lysates,
Lane 7: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PD-L1 antigen affinity purified polyclonal antibody (Catalog # PB9154) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002)？with Tanon 5200 system. A specific band was detected for PD-L1 at approximately 40-50 kDa. The expected band size for PD-L1 is at 33 kDa.
|
|
| 別品名 |
Programmed cell death 1 ligand 1;PD-L1;PDCD1 ligand 1;Programmed death ligand 1;B7 homolog 1;B7-H1;CD274;CD274;B7H1, PDCD1L1, PDCD1LG1, PDL1;
|
| 種由来 |
Human
|
| 交差種 |
Human
|
| 適用 |
Western Blot
Flow Cytometry
|
| 免疫動物 |
Rabbit
|
| 抗体クラス |
IgG
|
| 標識物 |
Cyanine 3
|
| 精製度 |
Affinity Purified
|
| GENE ID |
29126
|
| Accession No.(Gene/Protein) |
Q9NZQ7
|
| Gene Symbol |
CD274
|
| 分子量 |
33275 MW
|
| 概要 |
Boster Bio Anti-PD-L1/CD274 Antibody Picoband® catalog # PB9154. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
|
| 参考文献 |
1. Barber, D. L., Wherry, E. J., Masopust, D., Zhu, B., Allison, J. P., Sharpe, A. H., Freeman, G. J., Ahmed, R. Restoring function in exhausted CD8 T cells during chronic viral infection. Nature 439: 682-687, 2006.
2. Dong, H., Zhu, G., Tamada, K., Chen, L. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion.Nature Med. 5: 1365-1369, 1999.
3. Curiel, T. J., Wei, S., Dong, H., Alvarez, X., Cheng, P., Mottram, P., Krzysiek, R., Knutson, K. L., Daniel, B., Zimmermann, M. C., David, O., Burow, M., and 10 others. Blockade of B7-H1 improves myeloid dendritic cell-mediated antitumor immunity. Nature Med. 9: 562-567, 2003.
|
|
| メーカー |
品番 |
包装 |
|
BBT
|
PB9154-CY3
|
100 UG
|
※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
1週間程度
|
| 保存温度 |
-２０℃
|
|
