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Anti-TRAF2 antibody, PA2007, IHC(P)
IHC(P): Human Tonsil Tissue
Anti-TRAF2 antibody, PA2007, ICC
ICC: HELA Cell
Figure 1. Western blot analysis of TRAF2 using anti-TRAF2 antibody (PA2007).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysate,
Lane 2: rat PC-12 whole cell lysate,
Lane 3: mouse spleen tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF2 antigen affinity purified polyclonal antibody (Catalog # PA2007) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF2 at approximately 56KD. The expected band size for TRAF2 is at 56KD.
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Anti-TRAF2 antibody, PA2007, IHC(P)
IHC(P): Human Tonsil Tissue
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| 別品名 |
TNF receptor-associated factor 2;6.3.2.-;E3 ubiquitin-protein ligase TRAF2;Tumor necrosis factor type 2 receptor-associated protein 3;TRAF2;TRAP3;
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| 種由来 |
Human
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| 交差種 |
Human
Mouse
Rat
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| 適用 |
Western Blot
Immunohistochemistry
Immunocytochemistry (cell)
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 標識物 |
Fluorescein Isothiocyanate
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| 精製度 |
Affinity Purified
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| Accession No.(Gene/Protein) |
Q12933
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| Gene Symbol |
TRAF2
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| 分子量 |
55859 MW
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| 概要 |
Boster Bio Anti-TRAF2 Antibody catalog # PA2007. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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| 参考文献 |
1. Aizawa, S., Nakano, H., Ishida, T., Horie, R., Nagai, M., Ito, K., Yagita, H., Okumura, K., Inoue, J., Watanabe, T. Tumor necrosis factor receptor-associated factor (TRAF) 5 and TRAF2 are involved in CD30-mediated NF-kappa-B activation. J. Biol. Chem. 272: 2042-2045, 1997.
2. Kanamori, M., Suzuki, H., Saito, R., Muramatsu, M., Hayashizaki, Y. T2BP, a novel TRAF2 binding protein, can activate NF-kappa-B and AP-1 without TNF stimulation. Biochem. Biophys. Res. Commun. 290: 1108-1113, 2002.
3. Rothe, M., Xiong, J., Shu, H.-B., Williamson, K., Goddard, A., Goeddel, D. V. I-TRAF is a novel TRAF-interacting protein that regulates TRAF-mediated signal transduction. Proc. Nat. Acad. Sci. 93: 8241-8246, 1996.
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| メーカー |
品番 |
包装 |
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BBT
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PA2007-FITC
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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