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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of IDO2 using anti-IDO2 antibody (PA1612).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates (positive control),
Lane 2: human placenta tissue lysates (positive control),
Lane 3: human Caco-2 whole cell lysates (positive control),
Lane 4: human PC-3 whole cell lysates (negative control).
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDO2 antigen affinity purified polyclonal antibody (Catalog # PA1612) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDO2 at approximately 47KD. The expected band size for IDO2 is at 47KD.
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Figure 1. Western blot analysis of IDO2 using anti-IDO2 antibody (PA1612).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates (positive control),
Lane 2: human placenta tissue lysates (positive control),
Lane 3: human Caco-2 whole cell lysates (positive control),
Lane 4: human PC-3 whole cell lysates (negative control).
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDO2 antigen affinity purified polyclonal antibody (Catalog # PA1612) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDO2 at approximately 47KD. The expected band size for IDO2 is at 47KD.
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| 別品名 |
Indoleamine 2,3-dioxygenase 2;IDO-2;1.13.11.-;Indoleamine 2,3-dioxygenase-like protein 1;Indoleamine-pyrrole 2,3-dioxygenase-like protein 1;IDO2;INDOL1;
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| 種由来 |
Human
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| 交差種 |
Human
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| 適用 |
Western Blot
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 標識物 |
Cyanine 3
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| 精製度 |
Affinity Purified
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| Accession No.(Gene/Protein) |
Q6ZQW0
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| Gene Symbol |
IDO2
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| 分子量 |
45424 MW
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| 概要 |
Boster Bio Anti-INDOL1/IDO2 Antibody catalog # PA1612. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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| 参考文献 |
1. Ball, H. J., Sanchez-Perez, A., Weiser, S., Austin, C. J. D., Astelbauer, F., Miu, J., McQuillan, J. A., Stocker, R., Jermin, L. S., Hunt, N. H. Characterization of an indoleamine 2,3-dioxygenase-like protein found in humans and mice. Gene 396: 203-213, 2007.
2. Metz, R., DuHadaway, J. B., Kamasani, U., Laury-Kleintop, L., Muller, A. J., Prendergast, G. C. Novel tryptophan catabolic enzyme IDO2 is the preferred biochemical target of the antitumor indoleamine 2,3-dioxygenase inhibitory compound D-1-methyl-tryptophan. Cancer Res. 67: 7082-7087, 2007.
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| メーカー |
品番 |
包装 |
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BBT
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PA1612-CY3
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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