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Figure 1. Western blot analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (PA1015).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calretinin/CALB2 antigen affinity purified polyclonal antibody (Catalog # PA1015) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calretinin/CALB2 at approximately 29 kDa. The expected band size for Calretinin/CALB2 is at 29 kDa.
Figure 2. IHC analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (PA1015).
Calretinin/CALB2 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Calretinin/CALB2 Antibody (PA1015) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (PA1015).
Calretinin/CALB2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Calretinin/CALB2 Antibody (PA1015) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (PA1015).
Calretinin/CALB2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Calretinin/CALB2 Antibody (PA1015) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 5. IF analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (PA1015).
Calretinin/CALB2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Calretinin/CALB2 Antibody (PA1015) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLightR488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 1. Western blot analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (PA1015).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calretinin/CALB2 antigen affinity purified polyclonal antibody (Catalog # PA1015) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calretinin/CALB2 at approximately 29 kDa. The expected band size for Calretinin/CALB2 is at 29 kDa.
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| 別品名 |
Calretinin;CR;29 kDa calbindin;CALB2;CAB29;
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| 種由来 |
Human
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| 交差種 |
Human
Mouse
Rat
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| 適用 |
Western Blot
Immunohistochemistry
Immuno Fluorescence
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 標識物 |
Cyanine 3
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| 精製度 |
Affinity Purified
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| Accession No.(Gene/Protein) |
P22676
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| Gene Symbol |
CALB2
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| 分子量 |
31540 MW
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| 概要 |
Boster Bio Anti-Calretinin/CALB2 Antibody catalog # PA1015. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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| 参考文献 |
1. Parmentier M.; "The human calbindins: cDNA and gene cloning.";Adv. Exp. Med. Biol. 255:233-240(1989).
2. Strauss K.I., Kuznicki J., Winsky L., Kawagoe J.I., Hammer M., Jacobowitz D.M.;"The mouse calretinin gene promoter region: structural and functional components.";Brain Res. Mol. Brain Res. 49:175-187(1997).
3. Gabrielides C., McCormack A.L., Hunt D.F., Christakos S.;"Brain calbindin-D28k and an Mr 29,000 calcium binding protein in cerebellum are different but related proteins: evidence obtained from sequence analysis by tandem mass spectrometry.";Biochemistry 30:656-662(1991).
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| メーカー |
品番 |
包装 |
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BBT
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PA1015-CY3
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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