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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of UBR2 using anti-UBR2 antibody (M05812).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human K562 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-UBR2 antigen affinity purified monoclonal antibody (Catalog # M05812) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for UBR2 at approximately 202KD. The expected band size for UBR2 is at 202KD.
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Figure 1. Western blot analysis of UBR2 using anti-UBR2 antibody (M05812).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human K562 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-UBR2 antigen affinity purified monoclonal antibody (Catalog # M05812) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for UBR2 at approximately 202KD. The expected band size for UBR2 is at 202KD.
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| 別品名 |
Tubulin beta chain; Tubulin beta-5 chain; TUBB; TUBB5; OK/SW-cl.56
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| 種由来 |
Human
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| 交差種 |
Human
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| 適用 |
Western Blot
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| 免疫動物 |
Mouse
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| クローン |
2G10
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| 抗体クラス |
IgG2a
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| 標識物 |
Phycoerythrin
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| 精製度 |
Affinity Purified
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| GENE ID |
23304
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| Accession No.(Gene/Protein) |
Q8IWV8
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| Gene Symbol |
UBR2
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| 概要 |
Boster Bio Anti-UBR2 Picoband® Antibody (monoclonal, 2G10) catalog # M05812. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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| 参考文献 |
1. Kwon, Y. T., Xia, Z., An, J. Y., Tasaki, T., Davydov, I. V., Seo, J. W., Sheng, J., Xie, Y., Varshavsky, A. Female lethality and apoptosis of spermatocytes in mice lacking the UBR2 ubiquitin ligase of the N-end rule pathway. Molec. Cell. Biol. 23: 8255-8271, 2003.
2. Nagase, T., Ishikawa, K., Nakajima, D., Ohira, M., Seki, N., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 4: 141-150, 1997.
3. Yin, J., Kwon, Y. T., Varshavsky, A., Wang, W. RECQL4, mutated in the Rothmund-Thomson and RAPADILINO syndromes, interacts with ubiquitin ligases UBR1 and UBR2 of the N-end rule pathway. Hum. Molec. Genet. 13: 2421-2430, 2004.
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| メーカー |
品番 |
包装 |
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BBT
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M05812-PE
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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