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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of SYP using anti-SYP antibody (M05049-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat brain whole cell lysates,
Lane 3: mouse brain whole cell lysates,
Lane 4: mouse brain whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SYP antigen affinity purified polyclonal antibody (Catalog # M05049-3) at 0.5 g/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SYP at approximately 38KD. The expected band size for SYP is at 34KD.
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Figure 1. Western blot analysis of SYP using anti-SYP antibody (M05049-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat brain whole cell lysates,
Lane 3: mouse brain whole cell lysates,
Lane 4: mouse brain whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SYP antigen affinity purified polyclonal antibody (Catalog # M05049-3) at 0.5 g/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SYP at approximately 38KD. The expected band size for SYP is at 34KD.
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| 別品名 |
MRXSYP|Major synaptic vesicle protein p38
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| 種由来 |
Human
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| 交差種 |
Mouse
Rat
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| 適用 |
Western Blot
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| 免疫動物 |
Mouse
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| クローン |
3G12
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| 抗体クラス |
IgG2b
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| 標識物 |
Cyanine 3
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| 精製度 |
Affinity Purified
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| GENE ID |
6855
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| Accession No.(Gene/Protein) |
Human(P08247), Mouse(Q62277), Rat(P07825)
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| Gene Symbol |
SYP
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| 概要 |
Boster Bio Anti-SYP/Synaptophysin Antibody Picoband® (monoclonal, 3G12) catalog # M05049-3. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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| 参考文献 |
1. Calhoun ME, Jucker M, Martin LJ, Thinakaran G, Price DL, Mouton PR (December 1996). "Comparative evaluation of synaptophysin-based methods for quantification of synapses". J. Neurocytol.25 (12): 821-8.
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| メーカー |
品番 |
包装 |
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BBT
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M05049-3-CY3
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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