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Figure 1. Western blot analysis of Ankyrin erythroid/ANK/ANK1 using anti-Ankyrin erythroid/ANK/ANK1 antibody (M02716-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Ankyrin erythroid/ANK/ANK1 antigen affinity purified monoclonal antibody (Catalog # M02716-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Ankyrin erythroid/ANK/ANK1 at approximately 206 kDa. The expected band size for Ankyrin erythroid/ANK/ANK1 is at 206 kDa.
Figure 2. Flow Cytometry analysis of K562 cells using anti-Ankyrin erythroid/ANK/ANK1 antibody (M02716-1).
Overlay histogram showing K562 cells stained with M02716-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Ankyrin erythroid/ANK/ANK1 Antibody (M02716-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLightR488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 1. Western blot analysis of Ankyrin erythroid/ANK/ANK1 using anti-Ankyrin erythroid/ANK/ANK1 antibody (M02716-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Ankyrin erythroid/ANK/ANK1 antigen affinity purified monoclonal antibody (Catalog # M02716-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Ankyrin erythroid/ANK/ANK1 at approximately 206 kDa. The expected band size for Ankyrin erythroid/ANK/ANK1 is at 206 kDa.
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| 別品名 |
Histone-binding protein RBBP4; Chromatin assembly factor 1 subunit C; CAF-1 subunit C; Chromatin assembly factor I p48 subunit; CAF-I 48 kDa subunit; CAF-I p48; Nucleosome-remodeling factor subunit RBAP48; Retinoblastoma-binding protein 4; RBBP-4; Retinoblastoma-binding protein p48; RBBP4; RBAP48
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| 種由来 |
Human
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| 交差種 |
Human
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| 適用 |
Western Blot
Flow Cytometry
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| 免疫動物 |
Mouse
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| クローン |
5H2E8
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| 抗体クラス |
IgG2a
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| 標識物 |
Biotin
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| 精製度 |
Affinity Purified
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| GENE ID |
286
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| Accession No.(Gene/Protein) |
P16157
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| Gene Symbol |
ANK1
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| 概要 |
Boster Bio Anti-Ankyrin erythroid/ANK/ANK1 Antibody Picoband® (monoclonal, 5H2E8) catalog # M02716-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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| 参考文献 |
1. Davies, K. A., Lux, S. E. Hereditary disorders of the red cell membrane skeleton. Trends Genet. 5: 222-227, 1989.
2. Duru, F., Gurgey, A., Ozturk, G., Yorukan, S., Altay, C. Homozygosity for dominant form of hereditary spherocytosis. Brit. J. Haemat. 82: 596-600, 1992.
3. Eber, S. W., Gonzalez, J. M., Lux, M. L., Scarpa, A. L., Tse, W. T., Dornwell, M., Herbers, J., Kugler, W., Ozcan, R., Pekrun, A., Gallagher, P. G., Schroter, W., Forget, B. G., Lux, S. E. Ankyrin-1 mutations are a major cause of dominant and recessive hereditary spherocytosis. Nature Genet. 13: 214-218, 1996.
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| メーカー |
品番 |
包装 |
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BBT
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M02716-1-BIOTIN
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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