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Figure 1. Western blot analysis of SNRPN using anti-SNRPN antibody (M02173).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SNRPN antigen affinity purified monoclonal antibody (Catalog # M02173) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SNRPN at approximately 26KD. The expected band size for SNRPN is at 26KD.
Figure 2. Flow Cytometry analysis of A549 cells using anti-SNRPN antibody (M02173).
Overlay histogram showing A549 cells stained with M02173 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- SNRPN Antibody (M02173, 1μg/1x106 cells) for 30 min at 20°C. DyLightR488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 1. Western blot analysis of SNRPN using anti-SNRPN antibody (M02173).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SNRPN antigen affinity purified monoclonal antibody (Catalog # M02173) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SNRPN at approximately 26KD. The expected band size for SNRPN is at 26KD.
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| 別品名 |
Small nuclear ribonucleoprotein-associated protein N; snRNP-N; Sm protein D; Sm-D; Sm protein N; Sm-N; SmN; Tissue-specific-splicing protein; SNRPN; HCERN3; SMN
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| 種由来 |
Human
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| 交差種 |
Human
Mouse
Rat
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| 適用 |
Western Blot
Flow Cytometry
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| 免疫動物 |
Mouse
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| クローン |
6F12
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| 抗体クラス |
IgG2b
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| 標識物 |
Biotin
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| 精製度 |
Affinity Purified
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| GENE ID |
6638
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| Accession No.(Gene/Protein) |
P63162
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| Gene Symbol |
SNRPN
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| 概要 |
Boster Bio Anti-SNRPN Antibody Picoband® (monoclonal, 6F12) catalog # M02173. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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| 参考文献 |
1.Bielinska, B., Blaydes, S. M., Buiting, K., Yang, T., Krajewska-Walasek, M., Horsthemke, B., Brannan, C. I.?De novo deletions of?SNRPN?exon 1 in early human and mouse embryos result in a paternal to maternal imprint switch.?Nature Genet. 25: 74-78, 2000.2.Cattanach, B. M., Barr, J. A., Evans, E. P., Burtenshaw, M., Beechey, C. V., Leff, S. E., Brannan, C. I., Copeland, N. G., Jenkins, N. A., Jones, J.?A candidate mouse model for Prader-Willi syndrome which shows an absence of?Snrpn?expression.?Nature Genet. 2: 270-274, 1992.3.Geuns, E., De Rycke, M., Van Steirteghem, A., Liebaers, I.?Methylation imprints of the imprint control region of the?SNRPN-gene in human gametes and preimplantation embryos.?Hum. Molec. Genet. 12: 2873-2879, 2003.
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| メーカー |
品番 |
包装 |
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BBT
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M02173-BIOTIN
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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