| 別品名 |
Keratin, type II cytoskeletal 8; Cytokeratin-8; CK-8; K8; Type-II keratin Kb8; KRT8; CYK8
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| 種由来 |
Human
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| 標識物 |
DyLightTM 550
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| 精製度 |
Affinity Purified
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| 適用 |
Western Blot
Immunohistochemistry
Flow Cytometry
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| 免疫動物 |
Mouse
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| クローン |
6B7F8
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| 交差種 |
Human
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| GENE ID |
301
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| Accession No.(Gene/Protein) |
P04083
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| Gene Symbol |
ANXA1
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| 感度 |
>5000 cells
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| 形状 |
凍結乾燥品
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| 参考文献 |
1. Wallner BP, Mattaliano RJ, Hession C, Cate RL, Tizard R, Sinclair LK, Foeller C, Chow EP, Browing JL, Ramachandran KL (1986). "Cloning and expression of human lipocortin, a phospholipase A2 inhibitor with potential anti-inflammatory activity". Nature 320 (6057): 77?81.
2. "Entrez Gene: ANXA1 annexin A1".
3. Walther, A., Riehemann, K., Gerke, V. A novel ligand of the formyl peptide receptor: annexin I regulates neutrophil extravasation by interacting with the FPR. Molec. Cell 5: 831-840, 2000.
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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of Annexin A1/ANXA1 using anti-Annexin A1/ANXA1 antibody (M01451-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human U-87 MG whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Annexin A1/ANXA1 antigen affinity purified monoclonal antibody (Catalog # M01451-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Annexin A1/ANXA1 at approximately 35-39 kDa. The expected band size for Annexin A1/ANXA1 is at 39 kDa.
Figure 2. IHC analysis of Annexin A1/ANXA1 using anti-Annexin A1/ANXA1 antibody (M01451-3).
Annexin A1/ANXA1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Annexin A1/ANXA1 Antibody (M01451-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Figure 3. IHC analysis of Annexin A1/ANXA1 using anti-Annexin A1/ANXA1 antibody (M01451-3).
Annexin A1/ANXA1 was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Annexin A1/ANXA1 Antibody (M01451-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Figure 4. IHC analysis of Annexin A1/ANXA1 using anti-Annexin A1/ANXA1 antibody (M01451-3).
Annexin A1/ANXA1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Annexin A1/ANXA1 Antibody (M01451-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Figure 5. Flow Cytometry analysis of A549 cells using anti-Annexin A1/ANXA1 antibody (M01451-3). Overlay histogram showing A549 cells stained with M01451-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Annexin A1/ANXA1 Antibody (M01451-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 1. Western blot analysis of Annexin A1/ANXA1 using anti-Annexin A1/ANXA1 antibody (M01451-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human U-87 MG whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Annexin A1/ANXA1 antigen affinity purified monoclonal antibody (Catalog # M01451-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Annexin A1/ANXA1 at approximately 35-39 kDa. The expected band size for Annexin A1/ANXA1 is at 39 kDa.
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| メーカー |
品番 |
包装 |
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BBT
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M01451-3-DYLIGHT550
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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