| 別品名 |
E3 ubiquitin-protein ligase CHIP; Antigen NY-CO-7; CLL-associated antigen KW-8; Carboxy terminus of Hsp70-interacting protein; RING-type E3 ubiquitin transferase CHIP; STIP1 homology and U box-containing protein 1; STUB1; CHIP; PP1131
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| 種由来 |
Human
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| 標識物 |
DyLightTM 550
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| 精製度 |
Affinity Purified
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| 適用 |
Western Blot
Immunohistochemistry
Flow Cytometry
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| 免疫動物 |
Mouse
|
| クローン |
13I8
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| 交差種 |
Human
Mouse
Rat
Monkey
|
| GENE ID |
10273
|
| Accession No.(Gene/Protein) |
Q9UNE7
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| Gene Symbol |
STUB1
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| 感度 |
<10pg/ml
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| 形状 |
凍結乾燥品
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| 参考文献 |
1."Entrez Gene: STUB1 STIP1 homology and U-box containing protein 1".
2.Heimdal, K., Sanchez-Guixe, M., Aukrust, I., Bollerslev, J., Bruland, O., Jablonski, G. E., Erichsen, A. K., Gude, E., Koht, J. A., Erdal, S., Fiskerstrand, T., Haukanes, B. I., Boman, H., Bjorkhaug, L., Tallaksen, C. M. E., Knappskog, P. M., Johansson, S. STUB1 mutations in autosomal recessive ataxias--evidence for mutation-specific clinical heterogeneity. Orphanet J. Rare Dis. 9: 146, 2014.
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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of STUB1 using anti-STUB1 antibody (M01236).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: monkey COS-7 whole cell lysates,
Lane 3: human CACO-2 whole cell lysates.
Lane 4: human THP-1 whole cell lysates,
Lane 5: human PC-3 whole cell lysates,
Lane 6: human U87 whole cell lysates,
Lane 7: rat heart tissue lysates,
Lane 8: rat brain tissue lysates,
Lane 9: mouse brain tissue lysates,
Lane 10: mouse HEPA1-6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-STUB1 antigen affinity purified monoclonal antibody (Catalog # M01236) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for STUB1 at approximately 35KD. The expected band size for STUB1 is at 35KD.
Figure 2. IHC analysis of STUB1 using anti STUB1 antibody (M01236).
STUB1 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-STUB1 Antibody (M01236) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 3. IHC analysis of STUB1 using anti STUB1 antibody (M01236).
STUB1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-STUB1 Antibody (M01236) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 4. IHC analysis of STUB1 using anti STUB1 antibody (M01236).
STUB1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-STUB1 Antibody (M01236) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 5. IHC analysis of STUB1 using anti STUB1 antibody (M01236).
STUB1 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-STUB1 Antibody (M01236) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 6. IHC analysis of STUB1 using anti STUB1 antibody (M01236).
STUB1 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-STUB1 Antibody (M01236) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 7. Flow Cytometry analysis of A549 cells using anti- STUB1 antibody (M01236). Overlay histogram showing A549 cells stained with M01236 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-STUB1 Antibody (M01236, 1μg/1x106 cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 1. Western blot analysis of STUB1 using anti-STUB1 antibody (M01236).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: monkey COS-7 whole cell lysates,
Lane 3: human CACO-2 whole cell lysates.
Lane 4: human THP-1 whole cell lysates,
Lane 5: human PC-3 whole cell lysates,
Lane 6: human U87 whole cell lysates,
Lane 7: rat heart tissue lysates,
Lane 8: rat brain tissue lysates,
Lane 9: mouse brain tissue lysates,
Lane 10: mouse HEPA1-6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-STUB1 antigen affinity purified monoclonal antibody (Catalog # M01236) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for STUB1 at approximately 35KD. The expected band size for STUB1 is at 35KD.
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| メーカー |
品番 |
包装 |
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BBT
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M01236-DYLIGHT550
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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