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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of ZAP70 using anti-ZAP70 antibody (M00754-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human CCR-CEM whole cell lysates,
Lane 3: mouse spleen tissue lysates,
Lane 4: mouse thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ZAP70 antigen affinity purified monoclonal antibody (Catalog # M00754-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ZAP70 at approximately 70KD. The expected band size for ZAP70 is at 70KD.
Figure 2. Flow Cytometry analysis of 293T cells using anti-ZAP70 antibody (M00754-4).
Overlay histogram showing 293T cells stained with M00754-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti- ZAP70 Antibody (M00754-4, 1μg/1x106 cells) for 30 min at 20°C. DyLightR488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 1. Western blot analysis of ZAP70 using anti-ZAP70 antibody (M00754-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human CCR-CEM whole cell lysates,
Lane 3: mouse spleen tissue lysates,
Lane 4: mouse thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ZAP70 antigen affinity purified monoclonal antibody (Catalog # M00754-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ZAP70 at approximately 70KD. The expected band size for ZAP70 is at 70KD.
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| 別品名 |
Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase; ZAP70; SRK
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| 種由来 |
Human
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| 交差種 |
Human
Mouse
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| 適用 |
Western Blot
Flow Cytometry
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| 免疫動物 |
Mouse
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| クローン |
6F12
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| 抗体クラス |
IgG2b
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| 標識物 |
Fluorescein Isothiocyanate
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| 精製度 |
Affinity Purified
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| GENE ID |
7535
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| Accession No.(Gene/Protein) |
P43403
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| Gene Symbol |
ZAP70
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| 概要 |
Boster Bio Anti-ZAP70 Antibody Picoband® (monoclonal, 6F12) catalog # M00754-4. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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| 参考文献 |
1. Lupher ML, Reedquist KA, Miyake S, Langdon WY, Band H (Sep 1996). "A novel phosphotyrosine-binding domain in the N-terminal transforming region of Cbl interacts ly and selectively with ZAP-70 in T cells".The Journal of Biological Chemistry 271 (39): 24063-8.2. Meng W, Sawasdikosol S, Burakoff SJ, Eck MJ (Mar 1999). "Structure of the amino-terminal domain of Cbl complexed to its binding site on ZAP-70 kinase". Nature 398 (6722): 84-90.3. Neumeister EN, Zhu Y, Richard S, Terhorst C, Chan AC, Shaw AS (Jun 1995). "Binding of ZAP-70 to phosphorylated T-cell receptor zeta and eta enhances its autophosphorylation and generates specific binding sites for SH2 domain-containing proteins". Molecular and Cellular Biology 15 (6): 3171-8.
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| メーカー |
品番 |
包装 |
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BBT
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M00754-4-FITC
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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