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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of BAMBI using anti-BAMBI antibody (A07983-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAMBI antigen affinity purified polyclonal antibody (Catalog # A07983-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAMBI at approximately 29KD. The expected band size for BAMBI is at 29KD.
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Figure 1. Western blot analysis of BAMBI using anti-BAMBI antibody (A07983-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAMBI antigen affinity purified polyclonal antibody (Catalog # A07983-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAMBI at approximately 29KD. The expected band size for BAMBI is at 29KD.
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| 別品名 |
BMP and activin membrane-bound inhibitor homolog; Non-metastatic gene A protein; Putative transmembrane protein; NMA; BAMBI; NMA
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| 種由来 |
Human
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| 交差種 |
Human
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 標識物 |
Allophycocyanin
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| 精製度 |
Affinity Purified
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| GENE ID |
25805
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| Accession No.(Gene/Protein) |
Q13145
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| Gene Symbol |
BAMBI
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| 分子量 |
17136 MW
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| 概要 |
Boster Bio Anti-BAMBI/NMA Picoband® Antibody catalog # A07983-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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| 参考文献 |
1. Degen, W. G., Weterman, M. A., van Groningen, J. J., Cornelissen, I. M., Lemmers, J. P., Agterbos, M. A., Geurts van Kessel, A., Swart, G. W., Bloemers, H. P. Expression of nma, a novel gene, inversely correlates with the metastatic potential of human melanoma cell lines and xenografts. Int. J. Cancer 65: 460-465, 1996.
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| メーカー |
品番 |
包装 |
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BBT
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A07983-1-APC
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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