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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of TEAD3 using anti-TEAD3 antibody (A07748-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TEAD3 antigen affinity purified polyclonal antibody (Catalog # A07748-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TEAD3 at approximately 54 kDa. The expected band size for TEAD3 is at 49 kDa.
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Figure 1. Western blot analysis of TEAD3 using anti-TEAD3 antibody (A07748-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TEAD3 antigen affinity purified polyclonal antibody (Catalog # A07748-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TEAD3 at approximately 54 kDa. The expected band size for TEAD3 is at 49 kDa.
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| 別品名 |
Protein SOX-15; Protein SOX-12; Protein SOX-20; SOX15; SOX12; SOX20; SOX26; SOX27
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| 種由来 |
Human
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| 交差種 |
Mouse
Rat
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 標識物 |
Fluorescein Isothiocyanate
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| 精製度 |
Affinity Purified
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| GENE ID |
7005
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| Accession No.(Gene/Protein) |
Q99594
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| Gene Symbol |
TEAD3
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| 分子量 |
49229 MW
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| 参考文献 |
1. Jacquemin, P., Chen, Z., Martial, J. A., Davidson, I. Genomic structure and chromosomal mapping of the mouse transcription factor TEF-5 (Tead3) gene. Mammalian Genome 10: 632-634, 1999. 2. Jacquemin, P., Depetris, D., Mattei, M.-G., Martial, J. A., Davidson, I. Localization of human transcription factor TEF-4 and TEF-5 (TEAD2, TEAD3) genes to chromosomes 19q13.3 and 6p21.2 using fluorescence in situ hybridization and radiation hybrid analysis. Genomics 55: 127-129, 1999. 3. Jacquemin, P., Martial, J. A., Davidson, I. Human TEF-5 is preferentially expressed in placenta and binds to multiple functional elements of the human chorionic somatomammotropin-B gene enhancer. J. Biol. Chem. 272: 12928-12937, 1997.
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| メーカー |
品番 |
包装 |
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BBT
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A07748-2-FITC
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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