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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of SMARCD1 using anti-SMARCD1 antibody (A07318-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human PANC-1 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: human MOLT-4 whole cell lysates,
Lane 6: human Caco-2 whole cell lysates,
Lane 7: human SH-SY5Y whole cell lysates,
Lane 8: human Daudi whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMARCD1 antigen affinity purified polyclonal antibody (Catalog # A07318-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMARCD1 at approximately 58 kDa. The expected band size for SMARCD1 is at 58 kDa.
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Figure 1. Western blot analysis of SMARCD1 using anti-SMARCD1 antibody (A07318-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human PANC-1 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: human MOLT-4 whole cell lysates,
Lane 6: human Caco-2 whole cell lysates,
Lane 7: human SH-SY5Y whole cell lysates,
Lane 8: human Daudi whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMARCD1 antigen affinity purified polyclonal antibody (Catalog # A07318-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMARCD1 at approximately 58 kDa. The expected band size for SMARCD1 is at 58 kDa.
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| 別品名 |
Mesoderm posterior protein 1; Class C basic helix-loop-helix protein 5; bHLHc5; MESP1; BHLHC5
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| 種由来 |
Human
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| 交差種 |
Human
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| 適用 |
Western Blot
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 標識物 |
Cyanine 3
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| 精製度 |
Affinity Purified
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| GENE ID |
6602
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| Accession No.(Gene/Protein) |
Q96GM5
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| Gene Symbol |
SMARCD1
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| 分子量 |
108320 MW
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| 概要 |
Boster Bio Anti-SMARCD1 Antibody Picoband® catalog # A07318-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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| 参考文献 |
1. Nixon, K. C. J., Rousseau, J., Stone, M. H., Sarikahya, M., Ehresmann, S., Mizuno, S., Matsumoto, N., Miyake, N., DDD Study, Baralle, D., McKee, S., Izumi, K., Ritter, A. L., Heide, S., Heron, D., Depienne, C., Titheradge, H., Kramer, J. M., Campeau, P. M. A syndromic neurodevelopmental disorder caused by mutations in SMARCD1, a core SWI/SNF subunit needed for context-dependent neuronal gene regulation in flies. Am. J. Hum. Genet. 104: 596-610, 2019.
2. Ring, H. Z., Vameghi-Meyers, V., Wang, W., Crabtree, G. R., Francke, U. Five SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin (SMARC) genes are dispersed in the human genome. Genomics 51: 140-143, 1998.
3. Wang, W., Xue, Y., Zhou, S., Kuo, A., Cairns, B. R., Crabtree, G. R. Diversity and specialization of mammalian SWI/SNF complexes. Genes Dev. 10: 2117-2130, 1996.
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| メーカー |
品番 |
包装 |
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BBT
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A07318-1-CY3
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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