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Figure 1. Western blot analysis of TBP-1/PSMC3 using anti-TBP-1/PSMC3 antibody (A07208-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human HELA whole cell lysates,
Lane 3: human HEPG2 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: human U20S whole cell lysates,
Lane 6: human PC-3 whole cell lysates,
Lane 7: human U87 whole cell lysates,
Lane 8: rat stomach tissue lysates,
Lane 9: mouse lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBP-1/PSMC3 antigen affinity purified polyclonal antibody (Catalog # A07208-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TBP-1/PSMC3 at approximately 50KD. The expected band size for TBP-1/PSMC3 is at 50KD.
Figure 2. IHC analysis of TBP-1/PSMC3 using anti-TBP-1/PSMC3 antibody (A07208-1).
TBP-1/PSMC3 was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-TBP-1/PSMC3 Antibody (A07208-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of TBP-1/PSMC3 using anti-TBP-1/PSMC3 antibody (A07208-1).
TBP-1/PSMC3 was detected in paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-TBP-1/PSMC3 Antibody (A07208-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis of TBP-1/PSMC3 using anti-TBP-1/PSMC3 antibody (A07208-1).
TBP-1/PSMC3 was detected in paraffin-embedded section of human renal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-TBP-1/PSMC3 Antibody (A07208-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 5. IHC analysis of TBP-1/PSMC3 using anti-TBP-1/PSMC3 antibody (A07208-1).
TBP-1/PSMC3 was detected in paraffin-embedded section of human bladder tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-TBP-1/PSMC3 Antibody (A07208-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 6. IHC analysis of TBP-1/PSMC3 using anti-TBP-1/PSMC3 antibody (A07208-1).
TBP-1/PSMC3 was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-TBP-1/PSMC3 Antibody (A07208-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 7. Flow Cytometry analysis of 293T cells using anti-TBP-1/PSMC3 antibody (A07208-1).
Overlay histogram showing 293T cells stained with A07208-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TBP-1/PSMC3 Antibody (A07208-1, 1μg/1x106 cells) for 30 min at 20°C. DyLightR488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 1. Western blot analysis of TBP-1/PSMC3 using anti-TBP-1/PSMC3 antibody (A07208-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human HELA whole cell lysates,
Lane 3: human HEPG2 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: human U20S whole cell lysates,
Lane 6: human PC-3 whole cell lysates,
Lane 7: human U87 whole cell lysates,
Lane 8: rat stomach tissue lysates,
Lane 9: mouse lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBP-1/PSMC3 antigen affinity purified polyclonal antibody (Catalog # A07208-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TBP-1/PSMC3 at approximately 50KD. The expected band size for TBP-1/PSMC3 is at 50KD.
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| 別品名 |
DNA- (apurinic or apyrimidinic site) lyase 2;3.1.-.-;4.2.99.18;AP endonuclease XTH2;APEX nuclease 2;APEX nuclease-like 2;Apurinic-apyrimidinic endonuclease 2;AP endonuclease 2;APEX2;APE2, APEXL2, XTH2;
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| 種由来 |
Human
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| 交差種 |
Human
Mouse
Rat
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
Flow Cytometry
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 精製度 |
Affinity Purified
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| GENE ID |
5702
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| Accession No.(Gene/Protein) |
P17980
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| Gene Symbol |
PSMC3
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| 性状 |
Carrier free
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| 分子量 |
57401 MW
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| 概要 |
Boster Bio Anti-TBP-1/PSMC3 Antibody Picoband® catalog # A07208-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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| 参考文献 |
1. Corn, P. G., McDonald, E. R., III, Herman, J. G., El-Deiry, W. S. Tat-binding protein-1, a component of the 26S proteasome, contributes to the E3 ubiquitin ligase function of the von Hippel-Lindau protein. Nature Genet. 35: 229-237, 2003. 2. DeMartino, G. N., Proske, R. J., Moomaw, C. R., Strong, A. A., Song, X., Hisamatsu, H., Tanaka, K., Slaughter, C. A. Identification, purification, and characterization of a PA700-dependent activator of the proteasome. J. Biol. Chem. 271: 3112-3118, 1996. 3. Hoyle, J., Tan, K. H., Fisher, E. M. C. Localization of genes encoding two human one-domain members of the AAA family: PSMC5 (the thyroid hormone receptor-interacting protein, TRIP1) and PSMC3 (the Tat-binding protein, TBP1). Hum. Genet. 99: 285-288, 1997
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| メーカー |
品番 |
包装 |
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BBT
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A07208-1-CARRIER-FREE
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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