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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of SPARCL1 using anti-SPARCL1 antibody (A05726).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human SHG-44 whole cell lysates,
Lane 3: human MDA-MB-231 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse smooth muscle tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPARCL1 antigen affinity purified polyclonal antibody (Catalog # A05726) at 0.5mg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPARCL1 at approximately 62KD. The expected band size for SPARCL1 is at 75KD.
Figure 2. IHC analysis of SPARCL1 using anti-SPARCL1 antibody (A05726).
SPARCL1 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-SPARCL1 Antibody (A05726) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of SPARCL1 using anti-SPARCL1 antibody (A05726).
SPARCL1 was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-SPARCL1 Antibody (A05726) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 1. Western blot analysis of SPARCL1 using anti-SPARCL1 antibody (A05726).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human SHG-44 whole cell lysates,
Lane 3: human MDA-MB-231 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse smooth muscle tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPARCL1 antigen affinity purified polyclonal antibody (Catalog # A05726) at 0.5mg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPARCL1 at approximately 62KD. The expected band size for SPARCL1 is at 75KD.
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| 別品名 |
SPARC-like protein 1; High endothelial venule protein; Hevin; MAST 9; SPARCL1
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| 種由来 |
Human
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| 交差種 |
Human
Mouse
Rat
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| 適用 |
Western Blot
Immunohistochemistry
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 標識物 |
Fluorescein Isothiocyanate
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| 精製度 |
Affinity Purified
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| GENE ID |
8404
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| Accession No.(Gene/Protein) |
Q14515
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| Gene Symbol |
SPARCL1
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| 分子量 |
101272 MW
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| 概要 |
Boster Bio Anti-SPARCL1 Antibody Picoband® catalog # A05726. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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| 参考文献 |
1. Girard, J.-P., Springer, T. A. Cloning from purified high endothelial venule cells of hevin, a close relative of the antiadhesive extracellular matrix protein SPARC. Immunity 2: 113-123, 1995.
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| メーカー |
品番 |
包装 |
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BBT
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A05726-FITC
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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