| 別品名 |
Cytoskeleton-associated protein 5; Colonic and hepatic tumor overexpressed gene protein; Ch-TOG; CKAP5; KIAA0097
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| 種由来 |
Human
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| 標識物 |
DyLightTM 488
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| 精製度 |
Affinity Purified
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
Flow Cytometry
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| 免疫動物 |
Rabbit
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| 交差種 |
Human
Mouse
Rat
|
| GENE ID |
4140
|
| Accession No.(Gene/Protein) |
P27448
|
| Gene Symbol |
MARK3
|
| 分子量 |
39411 MW
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| 形状 |
凍結乾燥品
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| 参考文献 |
1. Ansar, M., Chung, H., Waryah, Y. M., Makrythanasis, P., Falconnet, E., Rao, A. R., Guipponi, M., Narsani, A. K., Fingerhut, R., Santoni, F. A., Ranza, E., Waryah, A. M., Bellen, H. J., Antonarakis, S. E. Visual impairment and progressive phthisis bulbi caused by recessive pathogenic variant in MARK3. Hum. Molec. Genet. 27: 2703-2711, 2018.
2. Lennerz, J. K., Hurov, J. B., White, L. S., Lewandowski, K. T., Prior, J. L., Planer, G. J., Gereau, R. W., IV, Piwnica-Worms, D., Schmidt, R. E., Piwnica-Worms, H. Loss of Par-1a/MARK3/C-TAK1 kinase leads to reduced adiposity, resistance to hepatic steatosis, and defective gluconeogenesis. Molec. Cell. Biol. 30: 5043-5056, 2010.
3. Muller, J., Ory, S., Copeland, T., Piwnica-Worms, H., Morrison, D. K. C-TAK1 regulates Ras signaling by phosphorylating the MAPK scaffold, KSR1. Molec. Cell 8: 983-993, 2001.
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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of MARK3 using anti-MARK3 antibody (A05355-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: rat NRK whole cell lysates,
Lane 4: mouse EL-4 whole cell lysates,
Lane 5: mouse 4T1 whole cell lysates,
Lane 6: mouse J774A.1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MARK3 antigen affinity purified polyclonal antibody (Catalog # A05355-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MARK3 at approximately 80 kDa. The expected band size for MARK3 is at 80 kDa.
Figure 2. IHC analysis of MARK3 using anti-MARK3 antibody (A05355-2).
MARK3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MARK3 Antibody (A05355-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Figure 3. IHC analysis of MARK3 using anti-MARK3 antibody (A05355-2).
MARK3 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MARK3 Antibody (A05355-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Figure 4. IHC analysis of MARK3 using anti-MARK3 antibody (A05355-2).
MARK3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MARK3 Antibody (A05355-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Figure 5. IHC analysis of MARK3 using anti-MARK3 antibody (A05355-2).
MARK3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MARK3 Antibody (A05355-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Figure 6. Flow Cytometry analysis of 293T cells using anti-MARK3 antibody (A05355-2).
Overlay histogram showing 293T cells stained with A05355-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MARK3 Antibody (A05355-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Figure 1. Western blot analysis of MARK3 using anti-MARK3 antibody (A05355-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: rat NRK whole cell lysates,
Lane 4: mouse EL-4 whole cell lysates,
Lane 5: mouse 4T1 whole cell lysates,
Lane 6: mouse J774A.1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MARK3 antigen affinity purified polyclonal antibody (Catalog # A05355-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MARK3 at approximately 80 kDa. The expected band size for MARK3 is at 80 kDa.
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| メーカー |
品番 |
包装 |
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BBT
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A05355-2-DYLIGHT488
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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