| 別品名 |
Fms-related tyrosine kinase 3 ligand ;Flt3lg ;
|
| 種由来 |
Human
|
| 精製度 |
Affinity Purified
|
| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
Flow Cytometry
|
| 免疫動物 |
Rabbit
|
| 交差種 |
Human
Mouse
Rat
|
| GENE ID |
5818
|
| Accession No.(Gene/Protein) |
Q15223
|
| Gene Symbol |
NECTIN1
|
| 添加剤 |
Carrier free
|
| 分子量 |
25847 MW
|
| 形状 |
凍結乾燥品
|
| 参考文献 |
1. Barron, M. J., Brookes, S. J., Draper, C. E., Garrod, D., Kirkham, J., Shore, R. C., Dixon, M. J. The cell adhesion molecule nectin-1 is critical for normal enamel formation in mice. Hum. Molec. Genet. 17: 3509-3520, 2008.
2. Geraghty, R. J., Krummenacher, C., Cohen, G. H., Eisenberg, R. J., Spear, P. G. Entry of alphaherpesviruses mediated by poliovirus receptor-related protein 1 and poliovirus receptor. Science 280: 1618-1620, 1998.
3. Lopez, M., Eberle, F., Mattei, M. G., Gabert, J., Birg, F., Bardin, F., Maroc, C., Dubreuil, P. Complementary DNA characterization and chromosomal localization of a human gene related to the poliovirus receptor-encoding gene. Gene 155: 261-265, 1995.
|
|
※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of PVRL1/NECTIN1 using anti-PVRL1/NECTIN1 antibody (A04988-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Hek293 whole cell lysates,
Lane 2: human CCRF-CEM whole cell lysates,
Lane 3: human Sw620 whole cell lysates,
Lane 4: human U87 whole cell lysates,
Lane 5: human SGC-7901 whole cell lysates,
Lane 6: human Jurkat whole cell lysates,
Lane 7: rat PC-12 whole cell lysates,
Lane 8: mouse spleen tissue lysates,
Lane 9: mouse NIH/3T3 whole cell lysates,
Lane 10: mouse Raw264.7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PVRL1/NECTIN1 antigen affinity purified polyclonal antibody (Catalog # A04988-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PVRL1/NECTIN1 at approximately 110KD. The expected band size for PVRL1/NECTIN1 is at 110KD.
Figure 2. IHC analysis of PVRL1/NECTIN1 using anti-PVRL1/NECTIN1 antibody (A04988-1).
PVRL1/NECTIN1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PVRL1/NECTIN1 Antibody (A04988-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of PVRL1/NECTIN1 using anti-PVRL1/NECTIN1 antibody (A04988-1).
PVRL1/NECTIN1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PVRL1/NECTIN1 Antibody (A04988-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis of PVRL1/NECTIN1 using anti-PVRL1/NECTIN1 antibody (A04988-1).
PVRL1/NECTIN1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PVRL1/NECTIN1 Antibody (A04988-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 5. IHC analysis of PVRL1/NECTIN1 using anti-PVRL1/NECTIN1 antibody (A04988-1).
PVRL1/NECTIN1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PVRL1/NECTIN1 Antibody (A04988-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 6. IHC analysis of PVRL1/NECTIN1 using anti-PVRL1/NECTIN1 antibody (A04988-1).
PVRL1/NECTIN1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PVRL1/NECTIN1 Antibody (A04988-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 7. Flow Cytometry analysis of PC-3 cells using anti-PVRL1/NECTIN1 antibody (A04988-1). Overlay histogram showing PC-3 cells stained with A04988-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PVRL1/NECTIN1 Antibody (A04988-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
|
|
|
|
Figure 1. Western blot analysis of PVRL1/NECTIN1 using anti-PVRL1/NECTIN1 antibody (A04988-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Hek293 whole cell lysates,
Lane 2: human CCRF-CEM whole cell lysates,
Lane 3: human Sw620 whole cell lysates,
Lane 4: human U87 whole cell lysates,
Lane 5: human SGC-7901 whole cell lysates,
Lane 6: human Jurkat whole cell lysates,
Lane 7: rat PC-12 whole cell lysates,
Lane 8: mouse spleen tissue lysates,
Lane 9: mouse NIH/3T3 whole cell lysates,
Lane 10: mouse Raw264.7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PVRL1/NECTIN1 antigen affinity purified polyclonal antibody (Catalog # A04988-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PVRL1/NECTIN1 at approximately 110KD. The expected band size for PVRL1/NECTIN1 is at 110KD.
|
|
|
| メーカー |
品番 |
包装 |
|
BBT
|
A04988-1-CARRIER-FREE
|
100 UG
|
※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
1週間程度
|
| 保存温度 |
-２０℃
|
|
