| 別品名 |
Mucosal addressin cell adhesion molecule 1; MAdCAM-1; hMAdCAM-1; MADCAM1
|
| 種由来 |
Human
|
| 標識物 |
DyLightTM 488
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| 精製度 |
Affinity Purified
|
| 適用 |
Western Blot
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| 免疫動物 |
Rabbit
|
| 交差種 |
Human
Rat
|
| GENE ID |
8174
|
| Accession No.(Gene/Protein) |
Q13477
|
| Gene Symbol |
MADCAM1
|
| 形状 |
凍結乾燥品
|
| 参考文献 |
1. Briskin, M. J., McEvoy, L. M., Butcher, E. C. MAdCAM-1 has homology to immunoglobulin and mucin-like adhesion receptors and to IgA1. Nature 363: 461-464, 1993.
2. Entrez Gene: mucosal vascular addressin cell adhesion molecule 1.
3. Leung, E., Berg, R. W., Langley, R., Greene, J., Raymond, L. A., Augustus, M., Ni, J., Carter, K. C., Spurr, N., Choo, K. H. A., Krissansen, G. W. Genomic organization, chromosomal mapping, and analysis of the 5-prime promoter region of the human MAdCAM-1 gene. Immunogenetics 46: 111-119, 1997.
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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of MADCAM1 using anti-MADCAM1 antibody (A04227-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: rat RH35 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MADCAM1 antigen affinity purified polyclonal antibody (Catalog # A04227-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MADCAM1 at approximately 50 kDa. The expected band size for MADCAM1 is at 40 kDa.
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Figure 1. Western blot analysis of MADCAM1 using anti-MADCAM1 antibody (A04227-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: rat RH35 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MADCAM1 antigen affinity purified polyclonal antibody (Catalog # A04227-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MADCAM1 at approximately 50 kDa. The expected band size for MADCAM1 is at 40 kDa.
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| メーカー |
品番 |
包装 |
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BBT
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A04227-3-DYLIGHT488
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100 UG
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※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
1週間程度
|
| 保存温度 |
-２０℃
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