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Figure 1. Western blot analysis of Myoglobin using anti-Myoglobin antibody (A04058).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: mouse heart tissue lysates,
Lane 4: mouse skeletal muscle tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Myoglobin antigen affinity purified polyclonal antibody (Catalog # A04058) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Myoglobin at approximately 17 kDa. The expected band size for Myoglobin is at 17 kDa.
Figure 2. IHC analysis of Myoglobin using anti-Myoglobin antibody (A04058).
Myoglobin was detected in a paraffin-embedded section of mouse skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Myoglobin Antibody (A04058) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Figure 3. IHC analysis of Myoglobin using anti-Myoglobin antibody (A04058).
Myoglobin was detected in a paraffin-embedded section of rat skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Myoglobin Antibody (A04058) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 1. Western blot analysis of Myoglobin using anti-Myoglobin antibody (A04058).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: mouse heart tissue lysates,
Lane 4: mouse skeletal muscle tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Myoglobin antigen affinity purified polyclonal antibody (Catalog # A04058) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Myoglobin at approximately 17 kDa. The expected band size for Myoglobin is at 17 kDa.
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| 別品名 |
Myoglobin;MB;
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| 種由来 |
Human
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| 交差種 |
Human
Mouse
Rat
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| 適用 |
Western Blot
Immunohistochemistry
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 標識物 |
Cyanine 3
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| 精製度 |
Affinity Purified
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| GENE ID |
4151
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| Accession No.(Gene/Protein) |
P02144
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| Gene Symbol |
MB
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| 分子量 |
17184 MW
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| 概要 |
Boster Bio Anti-Myoglobin/MB Antibody Picoband® catalog # A04058. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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| 参考文献 |
1. Boyer, S. H., Fainer, D. C., Naughton, M. A. Myoglobin inherited structural variation in man. Science 140: 1228-1231, 1963.
2. Boulton, F. E., Huntsman, R. G., Lorkin, P. A., Lehmann, H. Abnormal human myoglobin: 53(D4) glutamic acid lysine. Nature 223: 832-833, 1969.
3. Jeffreys, A. J., Wilson, V., Blanchetot, A., Weller, P., Geurts van Kessel, A., Spurr, N., Solomon, E., Goodfellow, P. The human myoglobin gene: a third dispersed globin locus in the human genome. Nucleic Acids Res. 12: 3235-3243, 1984.
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| メーカー |
品番 |
包装 |
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BBT
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A04058-CY3
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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