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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of HOMER1 using anti-HOMER1 antibody (A03877-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human COLO-320 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human A549 whole cell lysates,
Lane 6: human U-87MG whole cell lysates,
Lane 7: rat brain tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOMER1 antigen affinity purified polyclonal antibody (Catalog # A03877-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOMER1 at approximately 50 kDa. The expected band size for HOMER1 is at 50 kDa.
Figure 2. Flow Cytometry analysis of A549 cells using anti-HOMER1 antibody (A03877-1).
Overlay histogram showing A549 cells stained with A03877-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HOMER1 Antibody (A03877-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLightR488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 1. Western blot analysis of HOMER1 using anti-HOMER1 antibody (A03877-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human COLO-320 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human A549 whole cell lysates,
Lane 6: human U-87MG whole cell lysates,
Lane 7: rat brain tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOMER1 antigen affinity purified polyclonal antibody (Catalog # A03877-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOMER1 at approximately 50 kDa. The expected band size for HOMER1 is at 50 kDa.
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| 別品名 |
Transient receptor potential cation channel subfamily V member 3; TrpV3; Vanilloid receptor-like 3; VRL-3; TRPV3
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| 種由来 |
Human
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| 交差種 |
Human
Mouse
Rat
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 標識物 |
Cyanine 3
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| 精製度 |
Affinity Purified
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| GENE ID |
9456
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| Accession No.(Gene/Protein) |
Q86YM7
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| Gene Symbol |
HOMER1
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| 分子量 |
127459 MW
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| 参考文献 |
1. Ango, F., Prezeau, L., Muller, T., Tu, J. C., Xiao, B., Worley, P. F., Pin, J. P., Bockaert, J., Fagni, L. Agonist-independent activation of metabotropic glutamate receptors by the intracellular protein Homer. Nature 411: 962-965, 2001. 2. Bangash, M. A., Park, J. M., Melnikova, T., Wang, D., Jeon, S. K., Lee, D., Syeda, S., Kim, J., Kouser, M., Schwartz, J., Cui, Y., Zhao, X., and 10 others. Enhanced polyubiquitination of Shank3 and NMDA receptor in a mouse model of autism. Cell 145: 758-772, 2011. Note: Retraction: Cell 152: 367 only, 2013. 3. Brakeman, P. R., Lanahan, A. A., O'Brien, R., Roche, K., Barnes, C. A., Huganir, R. L., Worley, P. F. Homer: a protein that selectively binds metabotropic glutamate receptors. Nature 386: 284-288, 1997.
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| メーカー |
品番 |
包装 |
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BBT
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A03877-1-CY3
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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