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Figure 1. Western blot analysis of LIGHT/Tnfsf14 using anti-LIGHT/Tnfsf14 antibody (A03516-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse thymus tissue lysates,
Lane 2: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIGHT/Tnfsf14 antigen affinity purified polyclonal antibody (Catalog # A03516-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIGHT/Tnfsf14 at approximately 30 kDa. The expected band size for LIGHT/Tnfsf14 is at 26 kDa.
Figure 2. IHC analysis of LIGHT/Tnfsf14 using anti-LIGHT/Tnfsf14 antibody (A03516-2).
LIGHT/Tnfsf14 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LIGHT/Tnfsf14 Antibody (A03516-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Figure 3. IHC analysis of LIGHT/Tnfsf14 using anti-LIGHT/Tnfsf14 antibody (A03516-2).
LIGHT/Tnfsf14 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LIGHT/Tnfsf14 Antibody (A03516-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Figure 4. Flow Cytometry analysis of ANA-1 cells using anti-LIGHT/Tnfsf14 antibody (A03516-2).
Overlay histogram showing ANA-1 cells stained with A03516-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-LIGHT/Tnfsf14 Antibody (A03516-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLightR488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 1. Western blot analysis of LIGHT/Tnfsf14 using anti-LIGHT/Tnfsf14 antibody (A03516-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse thymus tissue lysates,
Lane 2: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIGHT/Tnfsf14 antigen affinity purified polyclonal antibody (Catalog # A03516-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIGHT/Tnfsf14 at approximately 30 kDa. The expected band size for LIGHT/Tnfsf14 is at 26 kDa.
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| 別品名 |
Tumor necrosis factor ligand superfamily member 14; CD258; Tumor necrosis factor ligand superfamily member 14, membrane form; Tumor necrosis factor ligand superfamily member 14, soluble form; Tnfsf14; Light;
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| 種由来 |
Mouse
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| 交差種 |
Mouse
Rat
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
Flow Cytometry
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 抗原部位 |
a.a.1-239
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| 精製度 |
Affinity Purified
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| GENE ID |
50930
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| Accession No.(Gene/Protein) |
Q9QYH9
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| Gene Symbol |
Tnfsf14
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| 性状 |
Carrier free
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| 概要 |
Boster Bio Anti-LIGHT/Tnfsf14 Antibody Picoband® catalog # A03516-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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| 参考文献 |
1. Doherty, T. A., Soroosh, P., Khorram, N., Fukuyama, S., Rosenthal, P., Cho, J. Y., Norris, P. S., Choi, H., Scheu, S., Pfeffer, K., Zuraw, B. L., Ware, C. F., Broide, D. H., Croft, M. The tumor necrosis factor family member LIGHT is a target for asthmatic airway remodeling. Nature Med. 17: 596-603, 2011.
2. Morel, Y., Schiano de Colella, J.-M., Harrop, J., Deen, K. C., Holmes, S. D., Wattam, T. A., Khandekar, S. S., Truneh, A., Sweet, R. W., Gastaut, J.-A., Olive, D., Costello, R. T. Reciprocal expression of the TNF family receptor herpes virus entry mediator and its ligand LIGHT on activated T cells: LIGHT down-regulates its own receptor. J. Immun. 165: 4397-4404, 2000.
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| メーカー |
品番 |
包装 |
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BBT
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A03516-2-CARRIER-FREE
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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