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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of LIGHT using anti-LIGHT antibody (A03516-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates,
Lane 2: mouse cardiac muscle tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIGHT antigen affinity purified polyclonal antibody (Catalog # A03516-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIGHT at approximately 26KD. The expected band size for LIGHT is at 26KD.
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Figure 1. Western blot analysis of LIGHT using anti-LIGHT antibody (A03516-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates,
Lane 2: mouse cardiac muscle tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIGHT antigen affinity purified polyclonal antibody (Catalog # A03516-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIGHT at approximately 26KD. The expected band size for LIGHT is at 26KD.
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| 別品名 |
Tumor necrosis factor ligand superfamily member 14; CD258; Tumor necrosis factor ligand superfamily member 14, membrane form; Tumor necrosis factor ligand superfamily member 14, soluble form; Tnfsf14; Light;
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| 種由来 |
Mouse
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| 交差種 |
Mouse
Rat
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 抗原部位 |
a.a.59-239
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| 標識物 |
Fluorescein Isothiocyanate
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| 精製度 |
Affinity Purified
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| GENE ID |
50930
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| Accession No.(Gene/Protein) |
Q9QYH9
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| Gene Symbol |
Tnfsf14
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| 概要 |
Boster Bio Anti-LIGHT/Tnfsf14 Antibody Picoband® catalog # A03516-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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| 参考文献 |
1. Doherty, T. A., Soroosh, P., Khorram, N., Fukuyama, S., Rosenthal, P., Cho, J. Y., Norris, P. S., Choi, H., Scheu, S., Pfeffer, K., Zuraw, B. L., Ware, C. F., Broide, D. H., Croft, M. The tumor necrosis factor family member LIGHT is a target for asthmatic airway remodeling. Nature Med. 17: 596-603, 2011.
2. Morel, Y., Schiano de Colella, J.-M., Harrop, J., Deen, K. C., Holmes, S. D., Wattam, T. A., Khandekar, S. S., Truneh, A., Sweet, R. W., Gastaut, J.-A., Olive, D., Costello, R. T. Reciprocal expression of the TNF family receptor herpes virus entry mediator and its ligand LIGHT on activated T cells: LIGHT down-regulates its own receptor. J. Immun. 165: 4397-4404, 2000.
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| メーカー |
品番 |
包装 |
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BBT
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A03516-1-FITC
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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