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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of Hemopexin/HPX using anti-Hemopexin/HPX antibody (A02237-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human HCCT tissue lysates,
Lane 3: human HCCP tissue lysates,
Lane 4: human placenta tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hemopexin/HPX antigen affinity purified polyclonal antibody (Catalog # A02237-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hemopexin/HPX at approximately 72 kDa. The expected band size for Hemopexin/HPX is at 72 kDa.
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Figure 1. Western blot analysis of Hemopexin/HPX using anti-Hemopexin/HPX antibody (A02237-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human HCCT tissue lysates,
Lane 3: human HCCP tissue lysates,
Lane 4: human placenta tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hemopexin/HPX antigen affinity purified polyclonal antibody (Catalog # A02237-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hemopexin/HPX at approximately 72 kDa. The expected band size for Hemopexin/HPX is at 72 kDa.
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| 別品名 |
T-cell surface glycoprotein CD8 alpha chain; CD8 antigen 32 kDa chain; OX-8 membrane antigen; CD8a
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| 種由来 |
Human
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| 交差種 |
Human
Mouse
Rat
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 抗原部位 |
a.a.97-447
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| 標識物 |
Phycoerythrin
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| 精製度 |
Affinity Purified
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| GENE ID |
3263
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| Accession No.(Gene/Protein) |
P02790
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| Gene Symbol |
HPX
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| 概要 |
Boster Bio Anti-Hemopexin/HPX Antibody Picoband® catalog # A02237-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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| 参考文献 |
1. Altruda, F., Poli, V., Restagno, G., Argos, P., Cortese, R., Silengo, L. The primary structure of human hemopexin deduced from cDNA sequence: evidence for internal, repeating homology. Nucleic Acids Res. 13: 3841-3859, 1985.
2. Altruda, F., Poli, V., Restagno, G., Silengo, L. Structure of the human hemopexin gene and evidence for intron-mediated evolution. J. Molec. Evol. 27: 102-108, 1988.
3. Cai, G.-Y., Law, M. L. Cloning and characterization of a human gene coding for hemopexin. (Abstract) Am. J. Hum. Genet. 39: A191 only, 1986.
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| メーカー |
品番 |
包装 |
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BBT
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A02237-2-PE
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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