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Figure 1. Western blot analysis of C-Jun/JUN using anti-C-Jun/JUN antibody (A02038-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human U-87MG whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C-Jun/JUN antigen affinity purified polyclonal antibody (Catalog # A02038-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C-Jun/JUN at approximately 36 kDa. The expected band size for C-Jun/JUN is at 36 kDa.
Figure 2. IHC analysis of C-Jun/JUN using anti-C-Jun/JUN antibody (A02038-3).
C-Jun/JUN was detected in a paraffin-embedded section of human renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-C-Jun/JUN Antibody (A02038-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Figure 3. IHC analysis of C-Jun/JUN using anti-C-Jun/JUN antibody (A02038-3).
C-Jun/JUN was detected in a paraffin-embedded section of human the renal pelvis squamous metaplasia tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-C-Jun/JUN Antibody (A02038-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Figure 4. IF analysis of C-Jun/JUN using anti-C-Jun/JUN antibody (A02038-3) and anti-Beta Tubulin antibody (M01857-3).
C-Jun/JUN was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-C-Jun/JUN beta Antibody (A02038-3) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLightR488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLightR594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Figure 5. Flow Cytometry analysis of U20S cells using anti-C-Jun/JUN antibody (A02038-3).
Overlay histogram showing U20S cells stained with A02038-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C-Jun/JUN Antibody (A02038-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLightR488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 1. Western blot analysis of C-Jun/JUN using anti-C-Jun/JUN antibody (A02038-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human U-87MG whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C-Jun/JUN antigen affinity purified polyclonal antibody (Catalog # A02038-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C-Jun/JUN at approximately 36 kDa. The expected band size for C-Jun/JUN is at 36 kDa.
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| 別品名 |
Short transient receptor potential channel 4; TrpC4; Trp-related protein 4; hTrp-4; hTrp4; TRPC4
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| 種由来 |
Human
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| 交差種 |
Human
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
Immuno Fluorescence
Immunocytochemistry (cell)
Flow Cytometry
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 抗原部位 |
a.a.35-331
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| 標識物 |
Horseradish Peroxidase
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| 精製度 |
Affinity Purified
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| GENE ID |
3725
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| Accession No.(Gene/Protein) |
P05412
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| Gene Symbol |
JUN
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| 分子量 |
37492 MW
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| 概要 |
Boster Bio Anti-c-Jun/JUN Antibody Picoband® catalog # A02038-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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| 参考文献 |
1. Aguilera, C., Nakagawa, K., Sancho, R., Chakraborty, A., Hendrich, B., Behrens, A. c-Jun N-terminal phosphorylation antagonises recruitment of the Mbd3/NuRD repressor complex. Nature 469: 231-235, 2011.
2. Bahary, N., Zorich, G., Pachter, J. E., Leibel, R. L., Friedman, J. M. Molecular genetic linkage maps of mouse chromosomes 4 and 6. Genomics 11: 33-47, 1991.
3. Behrens, A., Sibilia, M., Wagner, E. F. Amino-terminal phosphorylation of c-Jun regulates stress-induced apoptosis and cellular proliferation. Nature Genet. 21: 326-329, 1999.
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| メーカー |
品番 |
包装 |
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BBT
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A02038-3-HRP
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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