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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of NMDAR1 using anti-NMDAR1 antibody (A01808).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NMDAR1 antigen affinity purified polyclonal antibody (Catalog # A01808) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NMDAR1 at approximately 120KD. The expected band size for NMDAR1 is at 105KD.
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Figure 1. Western blot analysis of NMDAR1 using anti-NMDAR1 antibody (A01808).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NMDAR1 antigen affinity purified polyclonal antibody (Catalog # A01808) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NMDAR1 at approximately 120KD. The expected band size for NMDAR1 is at 105KD.
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| 別品名 |
Glutamate receptor ionotropic, NMDA 1; GluN1; Glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; NMD-R1; GRIN1; NMDAR1
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| 種由来 |
Human
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| 交差種 |
Human
Mouse
Rat
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| 適用 |
Western Blot
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 抗原部位 |
C-terminus
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| 標識物 |
Horseradish Peroxidase
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| 精製度 |
Affinity Purified
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| GENE ID |
2902
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| Accession No.(Gene/Protein) |
Q05586
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| Gene Symbol |
GRIN1
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| 概要 |
Boster Bio Anti-NMDAR1/GRIN1 Antibody Picoband® catalog # A01808. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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| 参考文献 |
1. Collins, C.; Duff, C.; Duncan, A. M. V.; Planells-Cases, R.; Sun, W.; Norremolle, A.; Michaelis, E.; Montal, M.; Worton, R.; Hayden, M. R. : Mapping of the human NMDA receptor subunit (NMDAR1) and the proposed NMDA receptor glutamate-binding subunit (NMDARA1) to chromosomes 9q34.3 and chromosome 8, respectively. Genomics 17: 237-239, 1993.
2. Karp, S. J.; Masu, M.; Eki, T.; Ozawa, K.; Nakanishi, S. : Molecular cloning and chromosomal localization of the key subunit of the human N-methyl-D-aspartate receptor. J. Biol. Chem. 268: 3728-3733, 1993.
3. Takano, H.; Onodera, O.; Tanaka, H.; Mori, H.; Sakimura, K.; Hori, T.; Kobayashi, H.; Mishina, M.; Tsuji, S. : Chromosomal localization of the epsilon-1, epsilon-3, and zeta-1 subunit genes of the human NMDA receptor channel. Biochem. Biophys. Res. Commun. 197: 922-926, 1993.
4. Zimmer, M.; Fink, T. M.; Franke, Y.; Lichter, P.; Spiess, J. : Cloning and structure of the gene encoding the human N-methyl-D-aspartate receptor (NMDAR1). Gene 159: 219-223, 1995.
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| メーカー |
品番 |
包装 |
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BBT
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A01808-HRP
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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