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Figure 1. Western blot analysis of CPS1 using anti-CPS1 antibody (A01320-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HCCT tissue lysates,
Lane 2: human HCCP tissue lysates,
Lane 3: monkey liver tissue lysates,
Lane 4: rat liver tissue lysates,
Lane 5: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPS1 antigen affinity purified polyclonal antibody (Catalog # A01320-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPS1 at approximately 165 kDa. The expected band size for CPS1 is at 165 kDa.
Figure 2. IHC analysis of CPS1 using anti-CPS1 antibody (A01320-2).
CPS1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CPS1 Antibody (A01320-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of CPS1 using anti-CPS1 antibody (A01320-2).
CPS1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CPS1 Antibody (A01320-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis of CPS1 using anti-CPS1 antibody (A01320-2).
CPS1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CPS1 Antibody (A01320-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 5. IF analysis of CPS1 using anti-CPS1 antibody (A01320-2).
CPS1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CPS1 Antibody (A01320-2) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Figure 6. Flow Cytometry analysis of Hela cells using anti-CPS1 antibody (A01320-2).
Overlay histogram showing Hela cells stained with A01320-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CPS1 Antibody (A01320-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLightR488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 1. Western blot analysis of CPS1 using anti-CPS1 antibody (A01320-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HCCT tissue lysates,
Lane 2: human HCCP tissue lysates,
Lane 3: monkey liver tissue lysates,
Lane 4: rat liver tissue lysates,
Lane 5: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPS1 antigen affinity purified polyclonal antibody (Catalog # A01320-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPS1 at approximately 165 kDa. The expected band size for CPS1 is at 165 kDa.
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| 別品名 |
Wee1-like protein kinase; WEE1hu; Wee1A kinase; WEE1
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| 種由来 |
Human
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| 交差種 |
Human
Mouse
Rat
Monkey
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
Immuno Fluorescence
Immunocytochemistry (cell)
Flow Cytometry
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 抗原部位 |
a.a.124-379
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| 標識物 |
Phycoerythrin
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| 精製度 |
Affinity Purified
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| GENE ID |
1373
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| Accession No.(Gene/Protein) |
P31327
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| Gene Symbol |
CPS1
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| 分子量 |
60066 MW
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| 概要 |
Boster Bio Anti-CPS1 Antibody Picoband® catalog # A01320-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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| 参考文献 |
1. Adcock, M. W., Ledbetter, D. H., O'Brien, W. E. Analysis of mammalian carbamyl phosphate synthetase I utilizing cDNA clones from human and rat liver. (Abstract) Fed. Proc. 43: 1726, 1984.
2. Adcock, M. W., O'Brien, W. E. Molecular cloning of cDNA for rat and human carbamyl phosphate synthetase I. J. Biol. Chem. 259: 13471-13476, 1984.
3. Aoshima, T., Kajita, M., Sekido, Y., Kikuchi, S., Yasuda, I., Saheki, T., Watanabe, K., Shimokata, K., Niwa, T. Novel mutations (H337R and 238-362del) in the CPS1 gene cause carbamoyl phosphate synthetase I deficiency. Hum. Hered. 52: 99-101, 2001.
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| メーカー |
品番 |
包装 |
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BBT
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A01320-2-PE
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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