|
※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of Chromogranin A/CHGA using anti-Chromogranin A/CHGA antibody (A01256-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Chromogranin A/CHGA antigen affinity purified polyclonal antibody (Catalog # A01256-4) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Chromogranin A/CHGA at approximately 70 kDa. The expected band size for Chromogranin A/CHGA is at 51 kDa.
Figure 2. IHC analysis of Chromogranin A/CHGA using anti-Chromogranin A/CHGA antibody (A01256-4).
Chromogranin A/CHGA was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Chromogranin A/CHGA Antibody (A01256-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Figure 3. IHC analysis of Chromogranin A/CHGA using anti-Chromogranin A/CHGA antibody (A01256-4).
Chromogranin A/CHGA was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Chromogranin A/CHGA Antibody (A01256-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Figure 4. IHC analysis of Chromogranin A/CHGA using anti-Chromogranin A/CHGA antibody (A01256-4).
Chromogranin A/CHGA was detected in a paraffin-embedded section of human testicular germ cell tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Chromogranin A/CHGA Antibody (A01256-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Figure 5. Flow Cytometry analysis of MCF-7 cells using anti-Chromogranin A/CHGA antibody (A01256-4).
Overlay histogram showing MCF-7 cells stained with A01256-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Chromogranin A/CHGA Antibody (A01256-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLightR488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
|
|
|
|
Figure 1. Western blot analysis of Chromogranin A/CHGA using anti-Chromogranin A/CHGA antibody (A01256-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Chromogranin A/CHGA antigen affinity purified polyclonal antibody (Catalog # A01256-4) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Chromogranin A/CHGA at approximately 70 kDa. The expected band size for Chromogranin A/CHGA is at 51 kDa.
|
|
| 別品名 |
Kit ligand; Mast cell growth factor; MGF; Stem cell factor; SCF; c-Kit ligand; Soluble KIT ligand; sKITLG; KITLG; MGF, SCF;
|
| 種由来 |
Human
|
| 交差種 |
Human
|
| 免疫動物 |
Rabbit
|
| 抗体クラス |
IgG
|
| 抗原部位 |
a.a.19-417
|
| 標識物 |
Phycoerythrin
|
| 精製度 |
Affinity Purified
|
| GENE ID |
1113
|
| Accession No.(Gene/Protein) |
P10645
|
| Gene Symbol |
CHGA
|
| 参考文献 |
1. Helman LJ, Ahn TG, Levine MA, Allison A, Cohen PS, Cooper MJ, Cohn DV, Israel MA (August 1988). Molecular cloning and primary structure of human chromogranin A (secretory protein I) cDNA. J. Biol. Chem. 263 (23): 11559-63. 2. Cotesta D, Caliumi C, Alo P, Petramala L, Reale MG, Masciangelo R, Signore A, Cianci R, D'Erasmo E, Letizia C (2005). High plasma levels of human chromogranin A and adrenomedullin in patients with pheochromocytoma. Tumori 91 (1): 53-8.
|
|
| メーカー |
品番 |
包装 |
|
BBT
|
A01256-4-PE
|
100 UG
|
※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
1週間程度
|
| 保存温度 |
-２０℃
|
|
※当社では商品情報の適切な管理に努めておりますが、表示される法規制情報は最新でない可能性があります。
また法規制情報の表示が無いものは、必ずしも法規制に非該当であることを示すものではありません。
商品のお届け前に最新の製品法規制情報をお求めの際はこちらへお問い合わせください。
|
※当社取り扱いの試薬・機器製品および受託サービス・創薬支援サービス（納品物、解析データ等）は、研究用としてのみ販売しております。
人や動物の医療用・臨床診断用・食品用としては、使用しないように、十分ご注意ください。
法規制欄に体外診断用医薬品と記載のものは除きます。
|
|
※リンク先での文献等のダウンロードに際しましては、掲載元の規約遵守をお願いします。
|
|
※CAS Registry Numbers have not been verified by CAS and may be inaccurate.
|
