| 別品名 |
E3 ubiquitin-protein ligase CHIP
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| 抗原部位 |
a.a.51-303
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| 種由来 |
Human
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| 標識物 |
DyLightTM 594
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| 精製度 |
Affinity Purified
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
Immuno Fluorescence
Immunocytochemistry (cell)
Flow Cytometry
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| 免疫動物 |
Rabbit
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| 交差種 |
Human
Mouse
Rat
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| GENE ID |
10273
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| Accession No.(Gene/Protein) |
Q9UNE7
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| Gene Symbol |
STUB1
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| 分子量 |
123799 MW
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| 形状 |
凍結乾燥品
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| 参考文献 |
1. "Entrez Gene: STUB1 STIP1 homology and U-box containing protein 1".
2. Heimdal, K., Sanchez-Guixe, M., Aukrust, I., Bollerslev, J., Bruland, O., Jablonski, G. E., Erichsen, A. K., Gude, E., Koht, J. A., Erdal, S., Fiskerstrand, T., Haukanes, B. I., Boman, H., Bjorkhaug, L., Tallaksen, C. M. E., Knappskog, P. M., Johansson, S. STUB1 mutations in autosomal recessive ataxias--evidence for mutation-specific clinical heterogeneity. Orphanet J. Rare Dis. 9: 146, 2014.
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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of STUB1 using anti-STUB1 antibody (A01236-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: rat pancreas tissue lysates,
Lane 6: mouse pancreas tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STUB1 antigen affinity purified polyclonal antibody (Catalog # A01236-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STUB1 at approximately 35 kDa. The expected band size for STUB1 is at 35 kDa.
Figure 2. IHC analysis of STUB1 using anti-STUB1 antibody (A01236-1).
STUB1 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-STUB1 Antibody (A01236-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of STUB1 using anti-STUB1 antibody (A01236-1).
STUB1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-STUB1 Antibody (A01236-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis of STUB1 using anti-STUB1 antibody (A01236-1).
STUB1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-STUB1 Antibody (A01236-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 5. IF analysis of STUB1 using anti-STUB1 antibody (A01236-1).
STUB1 was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-STUB1 Antibody (A01236-1) overnight at 4°C. DyLight?594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Figure 6. Flow Cytometry analysis of A549 cells using anti-STUB1 antibody (A01236-1).
Overlay histogram showing A549 cells stained with A01236-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STUB1 Antibody (A01236-1,1μg/1x106 cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Figure 1. Western blot analysis of STUB1 using anti-STUB1 antibody (A01236-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: rat pancreas tissue lysates,
Lane 6: mouse pancreas tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STUB1 antigen affinity purified polyclonal antibody (Catalog # A01236-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STUB1 at approximately 35 kDa. The expected band size for STUB1 is at 35 kDa.
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| メーカー |
品番 |
包装 |
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BBT
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A01236-1-DYLIGHT594
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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