| 抗原部位 |
a.a.1-811
|
| 種由来 |
Human
|
| 標識物 |
DyLightTM 550
|
| 精製度 |
Affinity Purified
|
| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
|
| 免疫動物 |
Rabbit
|
| 交差種 |
Human
|
| GENE ID |
8312
|
| Accession No.(Gene/Protein) |
O15169
|
| Gene Symbol |
AXIN1
|
| 形状 |
凍結乾燥品
|
| 参考文献 |
1. Chen, H.-J., Lin, C.-M., Lin, C.-S., Perez-Olle, R., Leung, C. L., Liem, R. K. H. The role of microtubule actin cross-linking factor 1 (MACF1) in the Wnt signaling pathway. Genes Dev. 20: 1933-1945, 2006.
2. Goto, T., Matsuzawa, J., Iemura, S., Natsume, T., Shibuya, H. WDR26 is a new partner of Axin1 in the canonical Wnt signaling pathway. FEBS Lett. 590: 1291-1303, 2016.
3. Huang, S.-M. A., Mishina, Y. M., Liu, S., Cheung, A., Stegmeier, F., Michaud, G. A., Charlat, O., Wiellette, E., Zhang, Y., Wiessner, S., Hild, M., Shi, X., and 24 others. Tankyrase inhibition stabilizes axin and antagonizes Wnt signalling. Nature 461: 614-620, 2009.
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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of AXIN1 using anti-AXIN1 antibody (A00986-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AXIN1 antigen affinity purified polyclonal antibody (Catalog # A00986-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AXIN1 at approximately 110 kDa. The expected band size for AXIN1 is at 96 kDa.
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|
|
Figure 1. Western blot analysis of AXIN1 using anti-AXIN1 antibody (A00986-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AXIN1 antigen affinity purified polyclonal antibody (Catalog # A00986-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AXIN1 at approximately 110 kDa. The expected band size for AXIN1 is at 96 kDa.
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|
| メーカー |
品番 |
包装 |
|
BBT
|
A00986-3-DYLIGHT550
|
100 UG
|
※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
1週間程度
|
| 保存温度 |
-２０℃
|
|
