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Figure 1. Western blot analysis of AXIN1 using anti-AXIN1 antibody (A00986-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HT-1080 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AXIN1 antigen affinity purified polyclonal antibody (Catalog # A00986-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AXIN1 at approximately 96,96-110 kDa. The expected band size for AXIN1 is at 96 kDa.
Figure 2. IF analysis of AXIN1 using anti-AXIN1 antibody (A00986-2) and anti-Beta Tubulin antibody (M01857-3).
AXIN1 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-AXIN1 Antibody (A00986-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLightR488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 1. Western blot analysis of AXIN1 using anti-AXIN1 antibody (A00986-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HT-1080 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AXIN1 antigen affinity purified polyclonal antibody (Catalog # A00986-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AXIN1 at approximately 96,96-110 kDa. The expected band size for AXIN1 is at 96 kDa.
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| 別品名 |
70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody
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| 抗原部位 |
a.a.534-792
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| 種由来 |
Human
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| 標識物 |
DyLightTM 488
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| 精製度 |
Affinity Purified
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immuno Fluorescence
Immunocytochemistry (cell)
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 交差種 |
Human
Mouse
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| GENE ID |
8312
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| Accession No.(Gene/Protein) |
O15169
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| Gene Symbol |
AXIN1
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| 分子量 |
52588 MW
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| 概要 |
Boster Bio Anti-AXIN1 Antibody Picoband® catalog # A06730-2. Tested in WB, ICC/IF, ELISA applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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| 形状 |
凍結乾燥品
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| 参考文献 |
1. Chen, H.-J., Lin, C.-M., Lin, C.-S., Perez-Olle, R., Leung, C. L., Liem, R. K. H. The role of microtubule actin cross-linking factor 1 (MACF1) in the Wnt signaling pathway. Genes Dev. 20: 1933-1945, 2006.
2. Goto, T., Matsuzawa, J., Iemura, S., Natsume, T., Shibuya, H. WDR26 is a new partner of Axin1 in the canonical Wnt signaling pathway. FEBS Lett. 590: 1291-1303, 2016.
3. Huang, S.-M. A., Mishina, Y. M., Liu, S., Cheung, A., Stegmeier, F., Michaud, G. A., Charlat, O., Wiellette, E., Zhang, Y., Wiessner, S., Hild, M., Shi, X., and 24 others. Tankyrase inhibition stabilizes axin and antagonizes Wnt signalling. Nature 461: 614-620, 2009.
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| メーカー |
品番 |
包装 |
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BBT
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A00986-2-DYLIGHT488
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100 UG
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※表示価格について
| 販売状況 |
長期B.O、納期未定
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| 当社在庫 |
なし
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| 納期目安 |
納期未定
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| 保存温度 |
-２０℃
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