|
※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of TRPM7 using anti-TRPM7 antibody (A00789-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysate,
Lane 2: human COLO-320 whole cell lysate,
Lane 3: human 22RV1 whole cell lysate,
Lane 4: human SGC-7901 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM7 antigen affinity purified polyclonal antibody (Catalog # A00789-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPM7 at approximately 250KD. The expected band size for TRPM7 is at 212KD.
Figure 2. IF analysis of TRPM7 using anti-TRPM7 antibody (A00789-1).
TRPM7 was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-TRPM7 Antibody (A00789-1) overnight at 4°C. DyLightR594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Figure 3. Flow Cytometry analysis of THP-1 cells using anti-TRPM7 antibody (A00789-1).
Overlay histogram showing THP-1 cells stained with A00789-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPM7 Antibody (A00789-1,1μg/1x106 cells) for 30 min at 20°C. DyLightR488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
|
|
|
|
Figure 1. Western blot analysis of TRPM7 using anti-TRPM7 antibody (A00789-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysate,
Lane 2: human COLO-320 whole cell lysate,
Lane 3: human 22RV1 whole cell lysate,
Lane 4: human SGC-7901 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM7 antigen affinity purified polyclonal antibody (Catalog # A00789-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPM7 at approximately 250KD. The expected band size for TRPM7 is at 212KD.
|
|
| 別品名 |
Transient receptor potential cation channel subfamily M member 7; Channel-kinase 1; Long transient receptor potential channel 7; LTrpC-7; LTrpC7; TRPM7; CHAK1; LTRPC7
|
| 種由来 |
Human
|
| 交差種 |
Human
Mouse
Rat
|
| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immuno Fluorescence
Immunocytochemistry (cell)
Flow Cytometry
|
| 免疫動物 |
Rabbit
|
| 抗体クラス |
IgG
|
| 抗原部位 |
a.a.777-905
|
| 標識物 |
Fluorescein Isothiocyanate
|
| 精製度 |
Affinity Purified
|
| GENE ID |
54822
|
| Accession No.(Gene/Protein) |
Q96QT4
|
| Gene Symbol |
TRPM7
|
| 分子量 |
59216 MW
|
| 概要 |
Boster Bio Anti-TRPM7 Antibody Picoband® catalog # A00789-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
|
| 参考文献 |
1. Aarts, M., Iihara, K., Wei, W.-L., Xiong, Z.-G., Arundine, M., Cerwinski, W., MacDonald, J. F., Tymianski, M. A key role for TRPM7 channels in anoxic neuronal death. Cell 115: 863-877, 2003.
2. Farooqi, A. A., Javeed, M. K., Javed, Z., Riaz, A. M., Mukhtar, S., Minhaj, S., Abbas, S., Bhatti, S.TRPM channels: same ballpark, different players, and different rules in immunogenetics.Immunogenetics 63: 773-787, 2011.
3. Hara, K., Kokubo, Y., Ishiura, H., Fukuda, Y., Miyashita, A., Kuwano, R., Sasaki, R., Goto, J., Nishizawa, M., Kuzuhara, S., Tsuji, S. TRPM7 is not associated with amyotrophic lateral sclerosis-parkinsonism dementia complex in the Kii peninsula of Japan. Am. J. Med. Genet. 153B: 310-313, 2010.
|
|
| メーカー |
品番 |
包装 |
|
BBT
|
A00789-1-FITC
|
100 UG
|
※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
1週間程度
|
| 保存温度 |
-２０℃
|
|
