| 別品名 |
Hepatitis A virus cellular receptor 2; HAVcr-2; T-cell immunoglobulin and mucin domain-containing protein 3; TIMD-3; T-cell immunoglobulin mucin receptor 3; TIM-3; T-cell membrane protein 3; HAVCR2; TIM3; TIMD3
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| 抗原部位 |
a.a.22-301
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| 種由来 |
Human
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| 精製度 |
Affinity Purified
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Flow Cytometry
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| 免疫動物 |
Rabbit
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| 交差種 |
Human
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| GENE ID |
84868
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| Accession No.(Gene/Protein) |
Q8TDQ0
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| Gene Symbol |
HAVCR2
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| 添加剤 |
Carrier free
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| 分子量 |
74585 MW
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| 形状 |
凍結乾燥品
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| 参考文献 |
1. "Entrez Gene: HAVCR2 hepatitis A virus cellular receptor 2".
2. Monney L, Sabatos CA, Gaglia JL, Ryu A, Waldner H, Chernova T, Manning S, Greenfield EA, Coyle AJ, Sobel RA, Freeman GJ, Kuchroo VK (Feb 2002). "Th1-specific cell surface protein Tim-3 regulates macrophage activation and severity of an autoimmune disease". Nature. 415 (6871): 536?41.
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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. Western blot analysis of TIM 3/HAVCR2 using anti-TIM 3/HAVCR2 antibody (A00657-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HCCP tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIM 3/HAVCR2 antigen affinity purified polyclonal antibody (Catalog # A00657-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIM 3/HAVCR2 at approximately 55 kDa. The expected band size for TIM 3/HAVCR2 is at 33 kDa.
Figure 2. Flow Cytometry analysis of HEL cells using anti-TIM 3/HAVCR2 antibody (A00657-4). Overlay histogram showing HEL cells stained with A00657-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TIM 3/HAVCR2 Antibody (A00657-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 1. Western blot analysis of TIM 3/HAVCR2 using anti-TIM 3/HAVCR2 antibody (A00657-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HCCP tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIM 3/HAVCR2 antigen affinity purified polyclonal antibody (Catalog # A00657-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIM 3/HAVCR2 at approximately 55 kDa. The expected band size for TIM 3/HAVCR2 is at 33 kDa.
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| メーカー |
品番 |
包装 |
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BBT
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A00657-4-CARRIER-FREE
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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