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Figure 1. Western blot analysis of C9ORF72 using anti-C9ORF72 antibody (A00419-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human U2OS whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human Hela whole cell lysates,
Lane 6: human A431 whole cell lysates,
Lane 7: human A549 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C9ORF72 antigen affinity purified polyclonal antibody (Catalog # A00419-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C9ORF72 at approximately 65KD. The expected band size for C9ORF72 is at 65KD.
Figure 2. Western blot analysis of C9ORF72 using anti-C9ORF72 antibody (A00419-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: mouse liver tissue lysates,
Lane 5: mouse HEPA1-6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C9ORF72 antigen affinity purified polyclonal antibody (Catalog # A00419-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C9ORF72 at approximately 65KD. The expected band size for C9ORF72 is at 65KD.
Figure 3. Flow Cytometry analysis of U20S cells using anti-C9ORF72 antibody (A00419-1).
Overlay histogram showing U20S cells stained with A00419-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C9ORF72 Antibody (A00419-1, 1μg/1x106 cells) for 30 min at 20°C. DyLightR488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 1. Western blot analysis of C9ORF72 using anti-C9ORF72 antibody (A00419-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human U2OS whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human Hela whole cell lysates,
Lane 6: human A431 whole cell lysates,
Lane 7: human A549 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C9ORF72 antigen affinity purified polyclonal antibody (Catalog # A00419-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C9ORF72 at approximately 65KD. The expected band size for C9ORF72 is at 65KD.
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| 別品名 |
Guanine nucleotide exchange C9orf72; C9orf72
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| 種由来 |
Human
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| 交差種 |
Human
Mouse
Rat
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| 適用 |
Western Blot
IHC paraffin embedding section
Flow Cytometry
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 抗原部位 |
N-terminus
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| 標識物 |
Cyanine 3
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| 精製度 |
Affinity Purified
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| GENE ID |
203228
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| Accession No.(Gene/Protein) |
Q96LT7
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| Gene Symbol |
C9ORF72
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| 分子量 |
14917 MW
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| 概要 |
Boster Bio Anti-C9ORF72 Antibody Picoband® catalog # A00419-1. Tested in Flow Cytometry, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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| 参考文献 |
1. DeJesus-Hernandez M, Mackenzie IR, Boeve BF, Boxer AL, Baker M, Rutherford NJ, Nicholson AM, Finch NA, Flynn H, Adamson J, Kouri N, Wojtas A, Sengdy P, Hsiung GY, Karydas A, Seeley WW, Josephs KA, Coppola G, Geschwind DH, Wszolek ZK, Feldman H, Knopman DS, Petersen RC, Miller BL, Dickson DW, Boylan KB, Graff-Radford NR, Rademakers R (2011). "Expanded GGGGCC hexanucleotide repeat in noncoding region of C9ORF72 causes chromosome 9p-linked FTD and ALS". Neuron. 72 (2): 245-56. 2. Babi? Leko, M; ?upunski, V; Kirincich, J; Smilovi?, D; Hortobagyi, T; Hof, PR; ?imi?, G (2019). "Molecular Mechanisms of Neurodegeneration Related to C9orf72 Hexanucleotide Repeat Expansion". Behavioural neurology. 2019: 2909168. doi:10.1155/2019/2909168.3. Bigio EH (2011). "C9ORF72, the new gene on the block, causes C9FTD/ALS: new insights provided by neuropathology". Acta Neuropathol. 122 (6): 653-5. doi:10.1007/s00401-011-0919-7.
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| メーカー |
品番 |
包装 |
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BBT
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A00419-1-CY3
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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