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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1. IHC analysis of Il2ra using anti-Il2ra antibody (A00214-2).
Il2ra was detected in a paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Il2ra Antibody (A00214-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Figure 2. IHC analysis of Il2ra using anti-Il2ra antibody (A00214-2).
Il2ra was detected in a paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Il2ra Antibody (A00214-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Figure 3. IF analysis of Il2ra using anti-Il2ra antibody (A00214-2).
Il2ra was detected in an immunocytochemical section of RAW264.7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Il2ra Antibody (A00214-2) overnight at 4°C. DyLightR488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 1. IHC analysis of Il2ra using anti-Il2ra antibody (A00214-2).
Il2ra was detected in a paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Il2ra Antibody (A00214-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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| 別品名 |
GTP-binding nuclear protein Ran;Androgen receptor-associated protein 24;GTPase Ran;Ras-like protein TC4;Ras-related nuclear protein;RAN;ARA24;OK/SW-cl.81;
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| 種由来 |
Mouse
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| 交差種 |
Mouse
Rat
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| 適用 |
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
Immuno Fluorescence
Immunocytochemistry (cell)
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 抗原部位 |
a.a.22-268
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| 標識物 |
Horseradish Peroxidase
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| 精製度 |
Affinity Purified
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| GENE ID |
16184
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| Accession No.(Gene/Protein) |
P01590
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| Gene Symbol |
Il2ra
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| 分子量 |
24423 MW
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| 概要 |
Boster Bio Anti-Il2ra Antibody Picoband® catalog # A00214-2. Tested in ELISA, IF, IHC, ICC applications. This antibody reacts with Mouse, Rat.
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| 参考文献 |
1. Ansano, M., Toda, M., Sakaguchi, N., Sakaguchi, S. Autoimmune disease as a consequence of developmental abnormality of a T cell subpopulation. J. Exp. Med. 184: 387-396, 1996.
2. Cosman, D., Cerretti, D. P., Larsen, A., Park, L., March, C., Dower, S., Gillis, S., Urdal, D. Cloning, sequence and expression of human interleukin-2 receptor. Nature 312: 768-771, 1984.
3. Hori, S., Nomura, T., Sakaguchi, S. Control of regulatory T cell development by the transcription factor Foxp3. Science 299: 1057-1061, 2003.
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| メーカー |
品番 |
包装 |
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BBT
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A00214-2-HRP
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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